为探索链霉菌(Streptomycete)702发酵液中菌丝体浸提条件,以链霉菌702发酵液中菌丝体及不同溶剂为实验材料,将菌丝体置于不同温度和不同pH下的有机溶剂中,对浸提时间及料液比进行优化。实验结果表明,90%以上的新农抗702存在于菌丝体中,可采用乙醇作为其浸提溶剂;当新农抗702在pH值6.5,料液比1∶2.0,温度30℃,时间12 h时其浸提效果最好;浸提完成时优化后的浸提液效价值较优化前高出约17%。

This study focused on the favorable extraction conditions of new agricultural antibiotic 702( new anti-702) from Streptomycete 702. The mycelium from fermentation broth of Streptomycete 702 and different solvents were used. The effects of extraction temperature,pH,extraction time and solid-liquid ratio on extraction efficiency were studied and the results of single factors were further optimized by orthogonal test. The results showed that over 90%new anti-702 was obtained from the mycelium of Streptomycete 702 and ethanol was the most preferred extraction solvent. The optimum extraction temperature,pH,extraction time and flesh-solvent ratio were 30 ℃,6. 5,12 h and1∶ 2. 0,respectively. Furthermore,under these conditions,the yield of new anti-702 was 17%. Therefore,this study provides a useful method for further study on the extraction and purification technology of the antifungal substance.