采用硫酸铵沉淀、离子交换层析、凝胶色谱等方法对麻鸭脂肪氧合酶(Shelduck Lipoxygenase)进行了纯化。结果显示,麻鸭LOX粗提液经60%~80%硫酸铵沉淀、DEAE离子交换层析、Superdex75凝胶过滤、SOURCE15Q离子交换层析后可达电泳纯,麻鸭LOX比活力为85714.3U/mg,纯化倍数98.64,经SDS PAGE分析麻鸭LOX的分子质量约为62 kDa。麻鸭LOX与大豆LOX的酶学性质差异较大,麻鸭LOX的最适温度为30℃,最适pH为6.0,且热稳定性和pH稳定性均优于大豆LOX。同时,Zn2+、Ca2+对麻鸭LOX的激活作用明显,而Mn2+对麻鸭LOX具有强烈的抑制作用。

Lipoxygenase from Shelduck muscle was purified by the combination of ammonium sulfate precipitation,anion exchange chromatography and gel filtration chromatography. The purified Shelduck LOX was electrophoretically pure and with the specific activity of 85 714. 3U / mg. Its molecular weight was about 62 kDa,which was smaller than that of the LOX from soybean. Further investigation revealed that the optimal temperature and pH value of shelduck LOX were 30℃ and 6. 0 respectively,which were quite different from that of Soybean LOX. The thermal and pH stability of Shelduck LOX were also better than that of soybean LOX. Zn2 +,Ca2 +had a strong activating effect on shelduck LOX,but Mn2 +was a strong inhibitor.