采用超临界CO2流体法萃取得到昆仑雪菊油树脂精油,用气相色谱-质谱联用(GC-MS)技术对其成分进行分离鉴定,结果表明:昆仑雪菊油树脂精油有84种化合物,主要成分为烯类(64.12%)、醇类(11.47%)、烷烃类(8.76%)、酸类(6.31%)、酯类(1.5%),占总化合物含量的92.16%。以抑菌圈和最低抑菌浓度MIC为考察指标,采用滤纸片法进行抑菌试验。发现昆仑雪菊油树脂精油对大肠杆菌、金黄色葡萄球菌、枯草杆菌、青霉、黑曲霉均有抑制作用。对大肠杆菌的最小抑菌浓度(MIC)值为25%,对金黄色葡萄球菌菌、枯草杆菌、青霉和黑曲霉的MIC值分别为50%、75%、50%和75%。

The Coreopsis oleoresin essential oil was extracted by supercritical CO 2 fluid,and the components were separated and identified by the gas chromatography mass spectrometry( GC-MS) technology. The results showed that 84 compounds in the essential oil from Coreopsis oleoresins were identified,and the main compounds amounted to 92. 16 % of their total compounds were olefins( 64. 12%),alcohols( 11. 47%),alkenes( 8. 76%),acids( 6. 31%),and esters( 1. 5%) which. The bacteriostatic circle and minimum inhibitory concentrations( MIC) was taken as the index for investigation. The antibacterial activity was evaluated by filter paper method. The results showed that Coreopsis oleoresin essential oil had significant inhibition effect on general fungi and bacteria. The minimum inhibitory concentration( MIC) on E. coli was 25%,and on Staphylococcus aureus,Bacillus subtilis,Penicilliumthe,Aspergillus niger were 50%,75%,50%,75%,respectively.