以匍枝根霉孢子为研究对象,通过改良固体培养法进行培养,并观察和统计匍枝根霉孢子的萌发情况、菌落大小和色泽、菌丝生长变化情况及孢子囊的形态特征,分析不同浓度山梨酸钾对抑制匍枝根霉生长的效果。结果表明:山梨酸钾对匍枝根霉具有显著的抑制作用,且抑制作用随其浓度升高而增强,当浓度≥0.8 g/100 mL时,抑菌效果已达到显著水平;当浓度为1.0~1.2 g/100 mL时,可抑制79%~90%根霉孢子的萌发,抑制菌丝及菌落生长且加速其老化、衰退。

This study aimed to analyze the inhibition of different concentration of potassium sorbate on the growth of poisonous Rhizopus stolonifer Vuill. The activated spores were cultured in modified solid culture medium which containing various concentrations of potassium sorbate,then spore germination number was counted,colony size was measured,and then colony color,the morphological characteristics of mycelium and hyphae were observed. The results showed that potassium sorbate significantly inhibit Rhizopus stolonifer Vuill,and the inhibitory effect was significantly related to the applied concentrations. When the concentration was equal or more than 0. 8 g /100mL,the inhibitory effect reached significant level. When the concentration was between 1. 0 g /100mL and 1. 2 g /100mL,79% ~ 90% spores could not germinate,colony and hypha grew slowly and even more,aged and decayed.