以海带为原料,研究前处理和双酶法糖化对海带中糖类物质溶出的影响,并对海带水解液进行乙醇初步发酵实验。实验结果表明:粒径为100目、浓度为5%(w/v)海带粉比较适合后续酶解糖化;用褐藻胶裂解酶和纤维素酶共同水解海带可以使其溶出较多糖类物质,约占海带干重的38%,其中甘露醇和可溶性总糖含量分别为17.8%和20.2%;利用毕赤酵母和棕榈发酵细菌发酵海带水解液,得到最大乙醇浓度分别为4.57 g/L(5.8mL/L)和2.68 g/L(3.4 mL/L),最大乙醇浓度达到理论转化率的28.99%。

The effects of acid pretreatment and enzymolysis with alginate lyase and cellulasedouble on extracting soluble sugars from L. japonica were investigated. The results showed that 100 mesh,5%( w / v) of kelp powder was suitable for successive saccharification via enzymatic hydrolysis using both alginate lyase and cellulase. Subsequently, the kelp extract with highest concentration of reducing sugars among all the treatments was applied for ethanol fermentation. Zymobacter palmae and Pichia angophorae were used as fermentative strains in fermentation of the kelp extract and the final ethanol yields were 4. 57g / L( 5. 8mL / L) and 2. 68g / L( 3. 4mL / L) respectively. The highest ethanol concentration of 4. 57g / L reached 28. 99% of the theortical yield.