以脱淀粉、脱蛋白的脱脂米糠为原料,对纤维素酶水解米糠提取阿魏酰低聚糖(FOs)的工艺进行了研究。在单因素实验的基础上,以FOs的提取量为响应值,采用响应面分析法对提取条件进行优化,得到最佳提取条件为:pH4.16,反应温度41.48℃,加酶量0.29 mg/g,底物浓度3.17%,反应时间11.76 h,在此条件下,FOs的提取量达到28.48μmol/g。米糠阿魏酰低聚糖对DPPH·和·OH均有一定的清除作用。其对DPPH·、·OH的EC50分别为0.750 5 mg/mL和3.270 5 mg/mL。

In order to improve the extraction efficiency of feruloyl oligosaccharides( FOs) from defatted rice bran using cellulase hydrolysis,single factor experiments and response surface analysis were employed to optimize the enzymolysis. The results suggested that the optimal extraction conditions were as follows: pH 4. 16,reaction temperature 55 ℃,enzyme addition amounts 0. 29 mg / g,and reaction time 11. 76 h. Under these conditions,the extraction amount of FOs reached 28. 48 μmol / g. And the scavenging effect of FOs on the DPPH · and · OH; EC50 were 0. 750 5 mg / mL and 3. 270 5 mg / mL,respectively.