建立高效液相色谱柱后衍生法测定鸡肉中的18种氨基酸。采用石油醚去除脂肪,盐酸水解处理样品,Lithium Ion-exchange锂离子交换色谱柱(3.0 mm×250 mm),流动相为锂离子酸性洗脱液Li175、锂离子中性洗脱液Li750、色谱柱再生液RG003的梯度洗脱。以TRIONE茚三酮为柱后衍生试剂,检测波长为570 nm。结果表明:18种氨基酸在8~80μg/mL内线性关系良好,回收率较高,同时分析了各类氨基酸在鸡肉中的比例。该检定方法稳定,同时适用于其他各种肉类产品中18种氨基酸的检测。

To develop a method for determination of eighteen amino acids by high performance liquid chromatography and post-column derivation in chicken.Methods petroleum ether was used to remove fat,hydrochloric acid was used to hydrolysis samples.The exchange chromatographic column( 3.0mm × 250mm) was Lithium Ion,mobile phase was Li175,Li750,RG003 as the gradient elution,TRIONE Ninhydrin was used for post-column derivative reagent,detection wavelength was 570 nm.Eighteen kinds of amino acids in 8 ~ 80 μg/mL range showed good linear relationship,recovery rate was high.The amino acid composition of the chicken was also analyzed.The method was simple,fast,accurate,high selectivity,high sensitivity,and stable.It is suitable for all kinds of other meat products and amino acid test.