以新鲜鸡冠为原料,采用乙醇法提取鸡冠中的透明质酸粗品,再用十六烷基三甲基溴化铵(CTAB)法对提出的粗品进行纯化。将纯化后的透明质酸用化学法进行降解,根据还原糖法测定降解后透明质酸的分子质量,进而确定降解程度。通过单因素试验和响应曲面法分析建立二次回归模型,对料液比、降解pH值、降解时间、过氧化氢浓度4个因素进行优化组合,确定降解鸡冠中透明质酸的佳工艺条件为:料液比(H2O2∶Vc,mol∶L)为4.13∶1、pH值为8.16、降解时间5 min、H2O2浓度为1.67 mol/L。经提取纯化得到的透明质酸的相对分子质量为22 671.88,经该工艺降解后得到的透明质酸的相对分子质量为1 764.67。

Hyaluronic acid was extracted from crude chicken comb by ethanol method and then purified by CTAB method.The degree of degradation was based on the reduced sugars content after hyaluronic acid degradation.Single factor experiment and response surface method analysis established two regression models to optimize the combination of the four factors of liquid material ratio,pH,degradation time,hydrogen peroxide concentration,The result of response surface experiment showed that the best condition was: ratio of material to liquid( H 2 O 2 ∶Vc) 4.13∶1,pH 8, the degradation time 5 min,and H 2 O 2 concentration 1.67 mol/L.The molecular weight of hyaluronic acid was 22671.88 after extraction and purification,molecular weight hyaluronic acid was 1764.67 under the above conditons.