以滤纸酶活为检测指标,通过单因素实验和均实验设计优化匍枝根霉产纤维素酶培养条件,并以此为基础进行分批发酵,并利用Logistic方程和Luedeking-Piret方程构建动力学模型。优化结果表明:滤纸酶活在发酵时间为84 h时达到13.16 IU/mL,比优化前提高了149%,其中,内切酶活、外切酶活和β-葡萄糖苷酶活高值分别为45.81 IU/mL、0.537 IU/mL和12.45 IU/mL且所建模型拟合良好。

In this experiment,the filter paper activity was used as index.The single factor experiment and uniform design optimization of medium and culture conditions were carried out.The batch fermentation was carried out on this basis using the Logistic equation and Luedeking-Piret equation establishes dynamic models.The results indicated that the filter paper activity reached 13.16 IU/mL at 84 h,which was increased 149% compared with that before optimization.The highest Endocellulases activity,Cellobiohydrolases activity and β-Glucosidases activity were 45.81 IU/mL,0.537 IU/mL,and 12.45 IU/mL,respectively.The model fitted well.