以Thielavia terrestris(ATCC38088)为出发菌株,通过单因素试验研究不同碳源(CMC-Na、Avicel、稻草粉、滤纸、麦麸、可溶性淀粉、葡萄糖)、不同氮源(牛肉膏、蛋白胨、氯化铵、黄豆饼粉、酵母提取物、硫酸铵)、不同的初始pH(3.0~6.0)等培养条件对该菌株滤纸酶活的影响。在此基础上,通过正交试验对该菌株产纤维素酶的条件进行了优化。结果表明,其产纤维素酶的佳条件为:以2.5%的麦麸为碳源、以2.0%的黄豆饼粉为氮源,初始pH值为4.0,在此条件下,45℃产酶发酵培养48 h,滤纸酶活力可达1.39 U/mL。

The effect of media components on filter paper activity of Thielavia terrestris( ATCC38088) was studied through single factor experiments,including different carbon sources( CMC-Na,Avicel,filter paper,straw powder,wheat bran,soluble starch,glucose),different nitrogen sources( beef extract,peptone,ammonium chloride, soybean meal,yeast extract,ammonium sulfate) and different initial pH value from 3.0 to 6.0.Based on the results of single factor experiments,liquid fermentation conditions for cellulase production of the strain were optimized through orthogonal test.The results showed that the optimum conditions were as follows: concentrations of straw powder and soybean meal were 2.5% and 2.0%,respectively.The initial pH value was 4.0.Under these conditions, the filter paper activity of the strain fermented at 45 ℃ for 48 h reached 1.39 U/mL.