采用响应面的方法优化了复合防腐剂对糟辣椒中腐败菌的佳抑菌配方。采用微量释法测定出三种防腐剂对从糟辣椒中分离出的13种腐败菌的小抑菌浓度MIC值,得到X2和X9作为实验菌;采用响应面优化法设计,以抑菌圈直径为响应值,对复合防腐剂进行复配。经响应面分析后建立多元回归模型,确定佳配方的添加量为:脱氢醋酸钠0.1 mg/mL,苯甲酸钠0.22 mg/mL,山梨酸钾0.14 mg/mL。

Optimization of composite preservative on Pickling Pepper products spoilage bacteria was studied through response surface methodology.The minimal inhibition concentration( MIC) of three preservatives for 13 kinds of spoilage bacteria was determined by microdilution method.X2and X9as the test bacteria,the preservatives formula of Pickling Pepper Products was optimized as bacteriostatic diameter of indicators.Multivariate regression model are built through response surface analysis,and optimal proportion of composite preservativ is that sodium acetate 0.1mg/mL,sodium benzoate 0.22mg/mL and potassium sorbate 0.14mg/mL.