对鞘氨醇单胞菌(Sphingomonas sp.)SC-1产3-苯氧基苯甲酸(3-PBA)降解酶的细胞定位分析,得出该降解酶主要位于细胞碎片上;用蔗糖密度梯度离心法分离浓缩细胞碎片中的细胞膜片段并检测其对3-PBA的降解,结果表明细胞膜片段具有定的3-PBA降解酶活性,推测该酶是类位于细胞膜上的膜蛋白;Native-PAGE分离细胞膜样品中的膜蛋白并切胶回收作对3-PBA的降解反应,条带c对3-PBA有定的降解活性,对条带c进行SDS-PAGE分析,推断出该降解酶的分子质量约为50~90 ku。

The cell localization analysis of 3- Phenoxybenzoic acid degrading-enzyme produced by Sphingomonas sp.SC-1 was studied in this article.The analysis showed that the degrading-enzyme was mainly located in the cell fragments.Separating the cell membrane fragments from concentration cell fragments by using sucrose density gradient centrifugation and detecting the effect on degrading 3-PBA.The result shows that the membrane fragments have some certain 3- phenoxybenzoic acid-degrading activity and it can be speculated that this enzyme is one kind of membrane protein on the cell membrane.Membrane protein with gel extraction separated from the sample by Native-PAGE was used to degrade 3- phenoxybenzoic acid.The band"c "had certain degrading activity.Using SDS-PAGE to analyze the band "c "and figure out that the molecular mass of degrading-enzyme is about 50 ~ 90 ku.