从金帅腐烂苹果中分离嗜酸耐热菌,进行形态学特征、生理生化特性、16S rDNA区鉴定,研究了温度、pH值对其生长的影响及芽孢耐热性。结果表明:分离得到了2株嗜酸耐热菌A-1与A-2,均为脂环酸芽孢杆菌属的酸土脂环酸芽孢杆菌,适生长温度均为45℃,适生长pH值分别为4.0、3.5;A-1、A-2芽孢的D90℃分别为11.90 min和13.70min,A-2表现出更强的耐热性。

In this paper,Alicyclobacillus acidoterrestris strains were isolated from Rotten Jinshua apples and identified by morphology,physiological and biochemical experiments,and 16S rDNA molecular analysis.The effects of temperature and pH on the growth and the heat resistance on their spores were also investigated.Their optimum growth temperatures were 45℃.The optimal pH values for A-1 and A-2 were 4.0 and 3.5,respectively.The D 90℃ values of the spores generated by A-1 and A-2 were 11.90 minutes and 13.70 minutes,respectively.A-2 indicated much stronger heat resistance as compared to A-1.