建立了一种简单、快速,同时检测水果中赤霉素、多效唑和烯效唑残留量的固相萃取-高效液相色谱(SPE-HPLC)方法。该方法经80%甲醇水提取样品,C18固相萃取小柱净化,采用DAD检测器220 nm紫外扫描检测。结果表明:3种植物生长调节剂在0.5~10μg/mL内线性关系良好(r≥0.999);检出限分别为赤霉素0.002 mg/kg、多效唑0.04 mg/kg、烯效唑0.02 mg/kg;在0.2~0.8 mg/kg加标水平下,3种植物生长调节剂平均回收率为78%~91%;相对标准偏差为2.4%~5.4%。

A simple and rapid SPE-HPLC method for the determination of Gibberellin,Paclobutrazol and Uniconazole residues in fruits was developed. The sample was extracted with 80% methanol-water solution,then was purified by C 18 column and detected in 220 nm with a DAD detector. Linear ranges of three plant growth regulators were in the range of 0. 5 ~ 10 μg / mL with correlation coefficients more than 0. 999. The minimum detection limit of Gibberellin,Paclobutrazol and Uniconazole were 0. 002 mg / kg,0. 04 mg / kg and 0. 02 mg / kg respectively. The average recoveries at spiked concentration levels of 0. 2 ~ 0. 8 mg / kg ranged from 78% to 91%,with relative standard deviations( RSDs) of 2. 4% ~ 5. 4%.