棉籽饼粉水解液作为廉价的有机氮源,在发酵领域中具有潜在的应用价值。对棉籽饼粉水解液和豆饼水解液中的氨基氮和氨基酸分析表明两种水解液的氨基氮含量分别为16.38 g/L和19.11 g/L,总氨基酸含量分别为97.78 g/L和91.59 g/L,两者营养成分接近。以L-谷氨酸发酵为例,考察了棉籽饼粉水解液和豆饼水解液作为有机氮源对谷氨酸温度敏感突变株强制发酵和生物素亚适量谷氨酸发酵工艺的影响。结果表明,在相同的发酵工艺下,分别以棉籽饼粉水解液和豆饼水解液作为有机氮源,最终得到的生物量以及L-谷氨酸产量相当。因此,棉籽饼粉水解液可以作为一种新型的有机氮源应用于发酵行业中,具有广阔的工业应用前景。

The cottonseed meal hydrolysate as nitrogen source with low price,had a potential value in the fermentation industry. The amino nitrogen concentration of cottonseed meal hydrolysate and soybean protein hydrolysate were 16. 38 g / L and 19. 11 g / L,respectively; and the total amino acid concentration were 97. 78 g / L and 91. 59 g / L,respectively,which indicated that the nutrients of the two hydrolysates were similar. In this study,the influence of the cottonseed meal hydrolysate and soybean protein hydrolysate as organic nitrogen sources on temperature triggered glutamate fermentation and biotin limitation glutamate fermentation were studied. Results showed that biomass and production of L-glutamate were similar by the two hydrolysates in the two types of glutamate fermentation. In conclusion,the cottonseed meal hydrolysate as an organic nitrogen source could provide practical values in the fermentation industry.