采用酶法制备了不同来源的Ⅱ型胶原,并对其理化性质及免疫活性进行了研究。结果表明:3种来源的软骨中均含有丰富的II型胶原,其中鸡胸软骨含量为81.54%,猪膝关节软骨含量为81.31%,羊肋软骨含量为80.94%;氨基酸组成分析和紫外吸收光谱鉴定表明,提取的胶原蛋白为典型II型胶原;SDS-PAGE电泳结果表明,所提取的II型胶原具有较高的纯度;傅立叶红外光谱和园二色光谱分析表明,3种来源的II型胶原在提取的过程中均保持着完整的二级结构;采用MTT法测定的淋巴细胞增殖反应结果表明,3种来源的II型胶原均具有免疫活性,且鸡胸软骨的活性最高,淋巴细胞增值率为52.91%。

Type II collagen was prepared by enzymatic method,and its physical-chemical properties and immunological activities were studied. The results showed that the cartilages from three different sources all contained abundant type II collagen. Chicken sternal cartilage had 81. 54%,pig swine knees cartilage had 81. 31%,and sheep cartilage had 80. 94% type II collagen. The amino acid component analysis and UV spectrum testing verified the extracted collagen was type II collagen. SDS-PAGE showed the extracts had a higher purity. Analysis by Infrared Spectrum and circular dichroism( CD) spectrogram revealed that the extracted type II collagen remained its completed secondary structures. Lymphocyte proliferative responses indicated that the extracted type II collagen possessed immunological activities. The immunological activity of collagen of chicken sternal cartilage was the highest,which attained 52. 91%.