研究了肠炎沙门氏菌(Salmonella enterica serovar Enteritidis,SE)在鲜切生菜清洗过程中的转移并建立转移模型,选择NaClO及复合抑菌剂(250IU/mL nisin+0.15%柠檬酸+0.05%双乙酸钠,nisin-CA-SD)为清洗剂,对比二者在控制SE转移能力上的差异,考察幂函数与指数函数2种模型在清洗环境中的适用性。研究结果表明,随着清洗次数的增加,SE从接种生菜转移到未接种生菜上的数量逐渐减少;NaClO及复合抑菌剂均不能完全抑制SE在清洗过程中的转移,但复合抑菌剂更有助于抑制SE的交叉污染。清洗剂多次利用时,幂函数模型能更好地描述及预测SE的转移情况。
The modeling of the transfer of Salmonella enterica serovar Enteritidis( SE) during washing operation of fresh-cut lettuce was investigated. NaClO and multiple antibacterial agents( 250IU / mL nisin + 0. 15% Citric acid + 0. 05% Sodium diacetate,nisin-CA-SD) were chosen as sanitizing agents,and their different abilities in controlling the transfer of SE were compared. The applicability of power function and exponential function of the cleaning environment were also studied. The results showed that the counts of SE transferred from inoculated lettuce to un-inoculated lettuce decreased when washing times increased. Both NaClO and multiple antibacterial agents could not totally inhibit the transfer of SE while washing. However,the use of multiple antibacterial agents helped to reduce cross-contamination of SE. Power function was appropriate to describe and predict the transfer of SE as sanitizing agents for repeated washing.