用聚酰胺层析法分离纯化牛蒡叶中总黄酮,并用分光光度法对其含量进行测定。文中采用水提、纤维素酶提以及纤维素酶乙醇提取方法,得到总黄酮含量不同的提取液,分别使之流经聚酰胺柱,其中的黄酮类物质被聚酰胺吸附后,用体积分数70%乙醇洗脱,考察洗脱曲线及纯度,采用分光光度法进行定量测定。结果显示:纤维素酶乙醇法提取液中黄酮含量最高,纤维素酶法次之,水提取法最低,70%乙醇可以将3种提取方法得到的绝大部分黄酮类化合物洗脱下来,回收率分别为93.0%、92.4%和93.7%,得到的产品纯度分别为15.84%、12.71%和12.33%。纤维素酶乙醇法可有效提高牛蒡叶中总黄酮的提取率,聚酰胺层析法可使总黄酮的纯度有较大幅度的提高。

To study separation and purification of total flavones in burdock leaves by polyamide column,and content was determined by spectrophotometry. Water extraction solvent,cellulase extraction solvent,cellulase and 70% alcohol extraction solvent from burdock leaves flew through polyamide column,then total flavones was absorbed and eluted by 70% alcohol,its solution eluting curve,purification and content were determined. The amount of total flavones was the best with cellulase and 70% alcohol. Next one was by cellulase only,the third was by water. Almost all of total flavonoid was eluted by 70% alcohol,the recovery rate of total flavones was 93. 0%,92. 4% and 93. 7% for three methods. And the purity were 15. 84%,12. 71% and 12. 33%,respectively. Extraction ratio of total flavones in burdock leaves raised by cellulase and alcohol extraction method,purification by polyamide column increased the purity dramatically. These data gave a valuable information for further research on burdock leaves.