文中对南极海茸多糖的理化性质、结构及其单糖组成进行了分析研究。南极海茸干品经脱脂、微波提取、Sevag法脱蛋白和分级醇沉得到海茸粗多糖,经过阴离子交换柱层析和凝胶层析分离得到均一多糖HRCS,采用HPLC、紫外全扫描、红外光谱扫描和气相色谱(GC)对海茸多糖结构进行光谱分析和单糖组成分析。结果表明:HRCS的分子质量为1.79×105Da,不含蛋白质和核酸,其中的单糖残基主要以α-构型和β-构型的吡喃环的形式存在,另外HRCS由L-岩藻糖、D-半乳糖和D-葡萄糖组成,摩尔比为2.18∶0.82∶1。

In this study,the chemical properties,structure and monosaccharide constituents of Durvillaea antarctica were analyzed. The polysaccharides were extracted by microwave and precipitated by ethanol after removing protein by Sevag method. Then the polysaccharides were separated and purified by ion exchange and gel filtration chromatography into uniform polysaccharide HRCS. The structure of Durvillaea antarctica polysaccharides and monosaccharide constituents were analyzed by HPLC,ultraviolet spectrum,infrared spectrum and gas chromatography. The result showed that HRCS with a molecular mass of 1. 79 × 105Da,contained no protein and nucleic acid,it mainly contained α-glycosidic bond and β- glycosidic bond with pyranose. Durvillaea antarctica polysaccharides HRCS contained 3 types of monosaccharide: L-fucose,D-galactose and D-glucose,their molar ratio was 2. 18∶ 0. 82∶ 1.