采用传统微生物学分离培养方法对我国海南西沙野生诺尼果内生菌进行分离与鉴定研究。计数结果显示,成熟野生诺尼果内生菌菌落总数达1.0×105CFU/g,其中真菌总数为8.0×103CFU/g。进一步在25~28℃下分离得到22株细菌、14株酵母菌和9株霉菌,分别采用16S rDNA、26S rDNA D1/D2区和ITS rDNA序列进行扩增和系统发育分析。结果表明,(1)22株内生细菌分属于α变形菌纲(α-Proteobacteria)、β变形菌纲(β-Proteobacteria)、γ变形菌纲(γ-Proteobacteria)、厚壁菌门(Firmicutes)和放线菌门(Actinobacteria)5个类群,包括亚西亚菌属(Asaia sp.)、黄杆菌属(Flavobacterium sp.)、伯克霍尔德氏菌属(Burkholderia sp.)、泛菌属(Pantoea sp.)、根瘤菌属(Rhizobium sp.)、类芽孢杆菌属(Paenibacillus sp.)、短小杆菌属(Curtobacterium sp.)、藤黄色杆菌属(Luteibacter sp.)8个属的10个已知种;(2)14株酵母分属于担子菌门(Basidiomycota)和子囊菌门(Ascomycota),包括隐球酵母菌属(Cryptococcus sp.)、Pseudozyma sp.、Sympodiomycopsis sp.和假囊酵母属(Eremothecium sp.)4个属的5个种;(3)9株霉菌均属于子囊菌门(Ascomycota),包括枝孢属(Cladosporium sp.)、轮层炭菌属(Daldinia sp.)、赤霉菌属(Gibberella sp.)、Nemania sp.和青霉属(Penicillium sp.)5个属的5个种。

The endophytes from the wild Noni fruit of Xisha was isolated and identified. Count results showed that the total endophytes' number of mature wild Noni fruit was 1. 0 × 105CFU/g and 8. 0 × 103CFU/g for the endophytic fungi. Furthermore,22 strains of bacteria,14 strains of yeast and 9 strains of mould were isolated. Partial sequence analysis of 16S rDNA,26S rDNA D1/D2 region and ITS rDNA were used for the strains' identification. The results indicated as follows:( 1) 22 strains of bacteria were highly similar to 8 known genus which belong to α-Proteobacteria,β-Proteobacteria, γ-Proteobacteria, Firmicutes and Actinobacteria. The 8 genus were Asaia sp., lavobacterium sp.,Burkholderia sp.,Pantoea sp.,Rhizobium sp.,Paenibacillus sp.,Curtobacterium sp. and Luteibacter sp.;( 2) 14 strains of yeast were highly similar to genus of Cryptococcus sp.,Pseudozyma sp.,Sympodiomycopsis sp. and Eremothecium sp. belonging to Basidiomycota and Ascomycota;( 3) 9 strains of mould were highly similar to genus of Cladosporium sp.,Daldinia sp.,Gibberella sp.,Nemania sp. and Penicillium sp. belonging to Ascomycota. The results showed that there are abundant species diversity of endophytes in the wild Noni fruit of Xisha. The results will lay the foundation for the research and development of functional strain's in Noni.