普通高温放线菌(Thermoactinomyces vulgaris)具有产生多种耐热酶的能力,在淀粉深加工、皮革制造、高温堆肥生产以及分子生物学等领域中均有重要应用,应用常规技术鉴别费时费力,无法满足普通高温放线菌筛选及其应用研究的要求,有必要建立一套快速、准确的鉴别方法。该研究根据普通高温放线菌的gyr B基因分别设计了普通高温放线菌的特异性引物,建立了快速筛选普通高温放线菌的特异PCR方法。实验结果表明所设计的特异性引物对普通高温放线菌具有较高的特异性,可以快速鉴别普通高温放线菌,与传统的菌种鉴定方法相比,具有高效、灵敏、便捷及成本低廉等显著优点。

Thermoactinomyces vulgaris can be applied to starch processing,leather manufacturing and high-temperature compost producing because it can produce various heat resistant enzymes. But identifying T. vulgaris by routine microbiological identification method is time-consuming and inconvenient,so it is essential to establish a rapid and accurate method for the identification of T. vulgaris. In this study one pair of specific primers were designed based on the gyrB gene of Thermoactinomyces vulgaris and a rapid specific PCR assay was established to screen T. vulgaris strains. Experimental results showed that the designed specific primers were specific for Thermoactinomyces vulgaris,and thus were suitable for rapidly screening T. vulgaris strains. The specific PCR method was proved to be more rapid and low-cost compared with traditional identifying methods.