在中性介质中,锰?对氧氟沙星的荧光强度具有显著的猝灭作用,基于此建立了一种测定锰?含量的荧光光谱分析法。在优化的实验条件下,测定了少量锰?加入反应体系前后的荧光强度,发现其荧光猝灭强度与锰?的浓度在2.5×10-7~1.0×10-5mol/L内呈现良好的线性关系,其检出限为8.4×10-8mol/L,相对标准偏差小于5%。该法应用于3种蔬菜样品中锰?含量的测定,结果较为满意。

Under neutral medium,manganese( II) could obviously quench the fluorescent intensity of levofloxacin. Based on this,a fluorescent spectral method for the detection of trace manganese( II) was developed. Under the optimal experiment conditions,fluorescent intensities with a small amount of manganese( II) and control were tested. It was found that the fluorescent quenching intensity was proportional to the concentration of manganese( Ⅱ) in the range of 2. 5 × 10-7~ 1. 0 × 10-5mol / L with the relative standard deviations of less than 5%. The limit of detection was as low as 8. 4 × 10-8mol / L( S / N = 3). This method was applied to detect the content of manganese( Ⅱ) in three vegetable samples with satisfactory results.