从杏鲍菇子实体中分离出纯化杏鲍菇子实体多糖,并对其结构进行研究。利用水提醇沉法从杏鲍菇子实体中提取粗多糖;采用离子柱层析和凝胶柱层析分段洗脱对其进行分离纯化,得到纯化的杏鲍菇子实体多糖PEPSC1,通过HPLC检测其为单一组分,分子质量为1.77×106Da;经糖组成分析、甲基化分析,PEPSC1由甘露糖和葡萄糖组成,摩尔比为1∶10.3,PEPSC1的结构主要以1,6葡萄糖为主链,葡萄糖为侧链,并且含有少量的1,6连接的甘露糖。

The purpose was to study on the structure of a homogeneous polysaccharide isolated from the fruiting bodies Pleurotus eryngii( PEPSC1). The PEPSC1 was isolated by hot water extraction and purified by DEAE Sepharose fast flow and Sephacryl S-500 column chromatography. The PEPSC1 was identified as a homogeneous composition by HPLC,and its molecular weight was 1. 77 × 106Da; Compositional analysis,combined with methylation analysis demonstrated that the PEPSC1 was composed of primarily mannose and glucose,their mole ratio was 1∶ 10. 3. PEPSC1 was structured by 1,6 linked glucose as backbone chain with glucose as the side chain. It contained a small amount of 1,6 linked mannose.