对油橄榄叶中的羟基酪醇进行提取及分离纯化。采用酶法对油橄榄叶中的羟基酪醇进行提取,大孔吸附树脂分离纯化。通过单因素实验和正交实验得到酶法提取羟基酪醇的最佳工艺为:酶解时间80 min,pH值为7,温度40℃,酶浓度为30μg/mL,在此条件下得到的羟基酪醇含量为3.72 mg/g。静态吸附实验筛选出DA-201大孔吸附树脂为分离纯化羟基酪醇的最佳树脂,动态吸附-脱附实验得到羟基酪醇分离纯化的最佳工艺为:上样液质量浓度为60μg/mL,上样流速为3 BV/h,脱附剂为体积分数80%的乙醇,脱附流速为3 BV/h,脱附剂用量为2 BV。按照以上最佳实验条件操作,1 g油橄榄叶可得到羟基酪醇总油状物为3.85 mg,纯度为87.01%。

The extraction and purification technology of hydroxytyrosol from olive leaves were studied. Hydroxytyrosol was obtainable by enzymolysis extraction and purified by macroporous adsorption resin. The single factor and orthogonal design were adopted to find out the optimal processing conditions. Results showed that the optimal enzymolysis conditions were determined as follows: pH 7,the enzyme concentration 30 μg / mL and extracting time 80 min at 40℃,the hydroxytyrosol content was 3. 72 mg / g. The experiments of static adsorption and desorption showed that DA-201 macroporous adsorption resin could adsorb and desorb hydroxytyrosol well,and the dynamic optimum technological conditions of the DA-201 were: the solution concentration at 60 μg / mL,the velocity of adsorption 3 BV / h, the eluting reagent 80% ethanol,the eluting velocity 3 BV / h,eluent volume at 2 BV. Under the above optimum experimental conditions,1g olive leaf can obtain 3. 85 mg hydroxytyrosol grease total saponin,the purity of hydroxytyrosol could reach 87. 01%. This method increased purity of hydroxytyrosol and set the foundation for the industrialized production.