通过静态吸附和解吸附筛选出最佳纯化树脂,通过动态吸附得出最佳纯化工艺。结果显示:X-5为龙葵果花色苷纯化的最佳吸附介质,其最优条件为:层析柱径长比为1∶25,最大上样量为0.6 BV,洗脱剂体积为3.5 BV,上样浓度为0.4 mg/mL,用pH 2.0的体积分数60%乙醇做洗脱剂,最适流速为2.0 BV/h。X-5树脂对龙葵果花色苷的纯化效果较好,纯化后的纯度为纯化前的19倍。

We selected the best macroporous resin through static adsorption and obtained the optimal purification conditions by the dynamic desorption. The results were as follows: X-5 macroporous resin exhibited the excellent characteristics for purification of Solanum nigrum L. anthocyanins. We found that the time of absorption and desorption equilibrium was 5 h and 6 h,respectively; the column diameter to length ratio was 1∶ 25,the maximum sample volume was 0. 6 BV,the concentration of absorption equilibrium was 0. 4 mg / mL,the eluent volume was 3. 5 BV, and elution flow rate was 2. 0 BV / h,60 % ethanol with pH 2. 0 was used as eluent. Under those optimal conditions, the purity was 19 times higher of unpurified ones. This research provides a theoretical base for deep processing of Solanum nigrum L. berries