文中收集了我国9个地区16个品种的蜂胶样品,采用体积分数95%乙醇萃取法和超临界CO2萃取法获得蜂胶萃取物,测定蜂胶得率和总黄酮含量,将数据统计分析并进行地区差异化研究。将2种萃取方法进行比较发现,超临界法萃取的蜂胶的总黄酮含量显著低于乙醇萃取法。地区化差异实验表明,河北和云南地区的蜂胶中总黄酮含量和得率普遍较高;湖北、山东、湖南和海南地区的蜂胶中总黄酮含量和得率居中;黑龙江地区的蜂胶中总黄酮含量和得率普遍较低;河南地区及四川地区内不同蜂胶品种间的总黄酮含量和得率差异极显著。

Two ethanol and supercritical carbon dioxide( CO2) extraction methods were used to produce propolis extracts from 9 districts and 16 varieties propolis samples in this study. The yield and total flavonoid content of propolis extracts were determined correspondingly. In addition,the propolis area differentiation was evaluated by data statistical analysis. By comparing two extraction methods,the results showed that the total flavonoid content of propolis extracts produced by the supercritical CO2extraction method was significantly lower than that produced by the ethanol extraction method. The propolis area differentiation results showed that the propolis grown in Hebei and Yunnan regions had generally higher yields and total flavonoid contents,the propolis developed at Hubei,Shandong,Hunan and Hainan areas had moderate yields and total flavonoid contents,and the propolis grown in Heilongjiang region had generally lower yield and total flavonoid content. Moreover,the total flavonoid contents of propolis extracts from different varieties grown in Henan and Sichuan regions showed significant differences. Therefore,this research suggested that the propolis production enterprises fixed the raw materials collecting area,variety and extraction method in order to guarantee the propolis product quality stability.