考察了利用氧化葡萄糖酸杆菌静息细胞转化乙二醇制备乙醇酸的工艺过程。在分批转化的条件下,100 g/L的乙二醇溶液经过30 h左右的反应,生成118.5 g/L的乙醇酸,转化率达到97%以上,静息细胞可以连续使用3个批次,平均转化率达到93.3%。通过补料分批转化,可以进一步的提高产量,经过38 h的反应得到182.5 g/L的乙醇酸,大大提高了转化液中的产物浓度。

The conversion of ethylene glycol to glycolic acid by resting cells of Gluconobacter oxydans was investigated. Under batch conversion conditions,118. 5 g /L of glycolic acid could get from ethylene glycol after 30 h of reaction. The conversion was above 97%. The resting cells could successively convert three cycles. The average conversion was about 93. 3%. By fed-batch conversion,the yield can be further improved. Over 38h reaction,182. 5 g /L of glycolic acid was obtained,which greatly increased the product concentration in the conversion liquor.