采用快速溶剂萃取法(ASE)及响应面分析法对香菇总生物碱提取工艺进行了优化;采用体外抗氧化活性法测定生物碱的总抗氧化能力、超氧阴离子和DPPH·自由基清除能力,评价其抗氧化活性。结果表明:ASE的最佳提取工艺为乙醇浓度80%,提取温度140℃,静态萃取20 min,提取2次,吹洗体积80%,具有较好的提取效率;香菇总生物碱具有较强的抗氧化性。

In this study,the alkaloids were extracted from Lentinula edodes via accelerated solvent extraction( ASE) and the response surface methodology( RSM) was used to optimize extraction conditions. The antioxidant activities were measured by DPPH,ABTS and superoxide anion radical scavenging capacity. ASE method can shorten the extraction time and improve extraction rate of total alkaloids. The results showed that the optimum conditions were: ethanol concentration 80%,extraction temperature at 140℃,static extraction time 20 min,extraction twice,blow volume fraction 80%. Under the above conditions,the alkaloids showed significant antioxidant activities.