在单因素实验的基础上,应用响应面法对芜菁多糖的提取工艺进行优化。测定了芜菁多糖清除自由基的活性。结果表明:芜菁多糖的最佳提取工艺为,浸提温度80℃,浸提时间2.5 h,液料比(30∶1)(mL∶g)。此条件下芜菁多糖的得率为8.858%,接近于理论值。芜菁多糖的体外清除自由基活性实验证明,芜菁多糖(WJc)对羟自由基的清除活性较好。

Response surface methodology( RSM) was used to optimize the extraction condition of crude polysaccharides( WJc) from Brassica rapa L.. Three independent variables of extraction temperature( ℃),extraction time( h),and ratio of water to raw material( mL/g) were investigated. The experimental levels were determined by singlefactor tests. The optimum conditions were as follows: extraction temperature 80℃,extraction time 2. 5 h,liquid /solid ratio 30 mL /g. Under these conditions,the experimental value was 8. 858%,which was very closed with the predicted valuel. In vitro,free radical scavenging activities results indicated that WJc were found to be effective in scavenging hydroxyl radicals. Overall,WJc may have potential applications in the medical and health food.