以红枣渣为原料,分别探讨了纤维素酶和木聚糖酶单酶加酶量、酶解时间、酶解温度、酶解pH对红枣渣可溶性和不溶性纤维得率的影响;在此基础上,以2种酶的添加量和酶解时间为变量,利用多因素复合试验设计原理和分析方法,得到枣渣纤维的酶法改性的最佳条件为:纤维素酶添加量0.29%、木聚糖酶添加量0.21%、酶解时间49.23 min。通过酶法改性,可将枣渣纤维的可溶性纤维的比例由6.79%提高到10.15%,以使可溶性纤维与不溶性纤维接近1∶3的最佳比例。

Jujube residue was used to discuss the enzymatic hydrolysis conditions of cellulase and xylanase respectively. On this basis,using the multi-factor composite test design principle and analysis method,the most suitable conditions to get the appropriate proportion of soluble fiber and insoluble fiber was determined,as two enzyme concentration and reaction time were variable. The optimized conditions were as the follows: cellulase 0. 29%,xylanase0. 21%,reaction time 49. 23 min. Under this condition,the proportion of soluble fiber could be increased from6. 79% to 10. 15% to achieve the best ratio of 1∶ 3 of soluble versus insoluble fiber.