建立了一种高效液相色谱分析方法,用于测定诺尼果汁车叶草苷酸的含量。采用Shim-pack VP-ODSC18(250 mm×4.6 mm,5μm)色谱柱,乙腈-冰醋酸水溶液为流动相,流速为1.0 min/mL,检测波长为230 nm。在此色谱条件下,车叶草苷酸分离度较好,浓度在5~50μg/mL内,线性关系良好,检测限为0.08μg/mL,加样回收率为99.75%,RSD值为2.25%。西沙诺尼果汁和大溪地诺尼果汁车叶草苷酸平均含量分别为0.218和0.179 mg/mL。

An analytical method of asperulosidic acid in Noni juice was established by HPLC. The separation was performed on a Shim-pack VP-ODS C18column( 250 mm × 4. 6 mm,5 μm) with acetonitrile-acetic acid aqueous as mobile phase at flow rate of 1. 0 mL /min and detection wave length at 230 nm. Asperulosidic acid was successfully separated. The calibration curve of asperulosidic acid was linear in concentration range of 5 ~ 50 μg /mL and the detection limit 0. 08 μg /mL. The average recoveries and RSD were 99. 75 % and 2. 25 %. The mean content of asperulosidic acid in Xisha and Tahiti Noni juice were 0. 218 and 0. 179 mg /mL.