为研究植物体内硒含量对辅酶Q含量的影响,以大豆为载体,通过叶面喷施亚硒酸钠进行富硒处理。采用原子荧光法测定大豆中硒含量,高效液相色谱法测定辅酶Q含量。考察了不同施硒浓度下大豆籽粒中硒和辅酶Q10含量。结果表明:随着施硒浓度的增加,大豆籽粒中硒累积量增加。硒含量由对照的78.89μg/kg,显著增加到345.12μg/kg;同时富硒大豆中籽粒中辅酶Q10含量也显著提高,且与硒含量呈线性关系,在研究的施硒浓度范围内,其线性方程为y=0.050 4x+2.374 8。与对照相比,施硒处理可以显著增加大豆籽粒中辅酶Q10含量,进而增加大豆的营养。

To study the effect of the content of selenium on coenzyme Q content in plants,Se-rich soybean was produced via Foliar applications of selenite. The content of selenium in soybean was determined by atomic fluorescence spectrometry,and content of coenzyme Q in soybean was determined by high performance liquid chromatography. The results showed that Se content was significantly increased in Se fertilizer sprayed soybean,from 78. 89 μg /kg to 345. 12 μg /kg. The effect of different concentrations of Selenium on the content of coenzyme Q10 in soybean was also studied. The result indicated that the content of coenzyme Q10 increased observably in Se-rich soybean. A linear relationship between the content of coenzyme Q10 and Se was discovered. The linear equation was y = 0. 0504 x +2. 3748. Therefore,Se-enriched treatment can rise the content of coenzyme Q10 in soybean. Therefore increase the nutrition of soybean.