从草菇子实体中提取水溶性粗多糖,经脱蛋白、脱色、DEAE-Sepharose F.F和Superdex-200柱层析,分离纯化得到一种水溶性草菇多糖组分VVP-1b。采用液相色谱、紫外光谱、红外光谱等光谱方法对其部分理化性质和结构进行测定。结果表明:VVP-1b为均一组分,具有明显的多糖特征峰,含有β-吡喃糖苷键;其总糖含量、蛋白含量、糖醛酸含量分别为88.7%、7.8%、27.6%,相对分子质量为5.84×105;单糖组成及摩尔比为阿拉伯糖∶甘露糖∶半乳糖∶葡萄糖=4.0∶1.0∶1.4∶35.4。因此VVP-1b是一种均一的具有β-吡喃糖苷键的酸性多糖。

A water-soluble polysaccharide(VVP-1b) was obtained by isolating and purifying from the fruiting bodies crude polysaccharides of Volvariella volvacea through deproteinization,decoloration,DEAE-Sepharose Fast Flow ion-exchange column chromatography and Superdex-200 column chromatography.Its partial properties and structure were studied by HPLC,UV,IR spectrum.The results showed that VVP-1b was a homogeneous polysaccharide with typical absorption of polysaccharide.β-glucosidic bond was determined by IR.The total sugar content of VVP-1b was 88.7%,the protein content of VVP-1b was 7.8% and uronic acid content was 27.6%.Its Mw was 5.84 × 105 Da.VVP-1b consists of Ara,Man,Gal,Glc,in the molar ratios of 4.0∶ 1.0∶ 1.4∶ 35.4.VVP-1b was a homogeneous acidic polysaccharide with β-pyranose glycosidic bond.