为了探讨使用酿酒曲发酵膨化木薯生产燃料乙醇的最优发酵条件,利用膨化技术对木薯进行了预处理,并用响应面法优化了发酵温度、液固比、糖化酶用量。结果表明:利用膨化处理缩短了乙醇生产周期,降低能源消耗,提高了生产效率。最适的发酵条件为:温度32℃、发酵初始pH值4.5、糖化酶用量145 U/g、液固比2.8∶1(mL∶g)、酿酒曲用量0.3%、发酵时间72 h。在此条件下,膨化木薯最终酒精度达到了11.94%。
关键词:
膨化; 木薯; 酿酒曲; 发酵; 响应面; 优化
In order to explore the optimal fermentation conditions of cassava for ethanol produc-tion using distiller's yeast,cassava were pretreated by extrusion technology in this paper,and the fermentation conditions including fermentation temperature,solid-liquid ratio,and enzyme concentration,were optimized by the response surface methodology.The results showed that extrusion technology could shorten the ethanol fermentation time,reduce energy consumption,and improve ethanol production efficiency.The optimal fermentation condition was as follows:temperature32 ℃,initial pH 4.5,enzyme dosage 145 U /g,liquid to solid ratio 2.8∶ 1(mL∶ g),distiller's yeast dosage 0.3%,and fermentation time 72 h.Under this condition,the final ethanol concentration from extruded cassava reached11.94 % Vol.