以甘蔗渣为原料,研究了甘蔗渣水解液的制备与利用琥珀酸放线杆菌发酵生产丁二酸的主要工艺参数。甘蔗渣经过1%NaOH预处理后,用纤维素酶、木聚糖酶、β-葡聚糖酶和果胶酶共同水解,在底物质量浓度为75 g/L时,水解液的还原糖质量浓度为65.6 g/L,甘蔗渣中总纤维素水解率达到90%。以还原糖质量浓度为55g/L的甘蔗渣水解液为碳源,在3 L发酵罐中分批发酵29 h产39.9 g/L丁二酸,丁二酸对甘蔗渣水解液中还原糖的得率为0.82 g/g,对甘蔗渣的得率为0.46 g/g。反映了以甘蔗渣为原料发酵生产丁二酸的可行性。

In this paper,a process for preparation of bagasse hydrolysate and production of succinic acid from bagasse by Actinobacillus succinogenes was studied.Bagasse pre-treated with 1% NaOH was degraded by complex enzymes comprising cellulase,xylanase,β-glucanase and pectinase.The reducing sugar concentration could be 65.6 g /L with a yield of 90% under the condition of 75 G /L substrate concentration.Using 55 g /L of bagasse hydrolysate as carbon source,fermentation was performed in a 3L stirred bioreactor,and 39.9 g /L succinic acid concentration was achieved with a succinic acid yield of 0.46 g /g(bagasse) at 29 h.The results showed the possibility of succinic acid production from bagasse.