毕赤酵母作为一种表达外源蛋白以及积累代谢中间物的细胞工厂已经得到广泛应用。文献检索是分析毕赤酵母研究的重要手段。该文基于Web of Science数据库分析了毕赤酵母的文献数量变化、来源国家分布等,以阐明我国有关毕赤酵母的研究状况。同时,展望了毕赤酵母外源基因表达技术、发酵策略、过氧化物酶体吞噬等新研究动向。

Pichia pastoris(P.pastoris) is a potential microbial cell factory for its high-level heterologous proteins production and metabolties accumulation.Literature analysis was a good tool to understand the situation about the whole P.pastoris research.Literature about P.pastoris from Web of Science database was organized according to publication time,countries,etc to show the situation of China in this field.At the same time,the emerging research opportunities,such as genetic manipulation,fermentation strategies,pexophagy,were reviewed for research work at next stage.