以库尔勒香梨为试验材料,采用1-甲基环丙烯(1-MCP)处理,研究其对库尔勒香梨在常温贮藏过程中对活性氧相关代谢的影响,探讨1-MCP处理对库尔勒香梨衰老的影响。库尔勒香梨经1μL/L 1-MCP处理后,放置在室温(20±1)℃、80%~85%RH的条件下,对果实中活性氧超氧阴离子自由基(O2-·)、过氧化氢(H2O2)含量及代谢的相关酶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性和丙二醛(MDA)含量进行测定。结果表明:常温贮藏下,1μL/L 1-MCP处理能够延缓香梨果实CAT、POD活性的下降,保持SOD酶活性,降低O2-·产生和H2O2积累,抑制膜脂过氧化产物丙二醛的积累,从而减少活性氧对细胞膜的破坏,延缓了香梨果实采后衰老。

The effect of treatment with 1-MCP on reactive oxygen species(ROS) metabolism on Korle fragrant pear at room temperature was studied.The fresh-keeping effect of the treatment with 1-MCP at room temperature provided a theoretical basis for room temperature storage of Korle fragrant pear.After treating with 1 μL /L 1-MCP,pears were then stored at(20 ± 1) ℃ and 80% ~ 85% RH.The reactive oxygen species metabolism related enzymes were determined on O2-.production rate,hydrogen peroxide(H2O2) content,malondialdehyde(MDA) and the activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD).The results showed that fruit treated with 1μL/L 1-MCP could delay the declining of CAT and POD activity,maintain SOD activity,decrease the generation of O2-.and accumulation of H2O2 content,inhibit the accumulation of membrane lipid peroxidation product malondialdehyde.The oxygen damage to the cell membrane was reduced,and therefore,senescence during storage at room temperature in Korle fragrant pear was delayed.