研究了牡蛎来源的1株具有较高抑制乙酰胆碱酯酶活性的共生真菌的最佳发酵条件,并采用形态学和分子生物学方法对其进行了鉴定。菌株WFW mL4为球毛壳菌,命名为Chaetomium globosum WFW mL4;其发酵的最佳条件为:SP培养基,初始pH值为6.0,接种量10%,500 mL三角瓶装液量200 mL,在25℃发酵10 d,摇床转速120 r/min。此条件下获得的发酵液粗提物在5 mg/mL时,对乙酰胆碱酯酶(AChE)抑制率可达到(68.07±3.23)%,光照对该菌株几乎无影响。牡蛎共生真菌的次生代谢产物具有较好的抑制AChE的活性,为抗老年痴呆药物的开发提供了新的来源。

A symbiotic fungi WFWML4 with acetylcholinesterase-inhibiting activity from Crassostrea rivularis was identified and its fermentation conditions were optimized.The strain was identified by both microscopic morphology and analysis of the nucleotide sequence of the 18 S rDNA.And fermentation medium,time,temperature,pH value,inoculum concentration,light period and culture volume were optimized to improve the inhibition activity of AChE in fermentation broth.Results showed the strain was confirmed as Chaetomium globosum,and the optimal fermentation conditions were as follows:SP medium was the suitable medium for the strain at pH 6.0.The light period was not a major factor during the AChE inhibitor production.The strain WFWML4 exhibited a higher AChE inhibitory activity68.07% ± 3.23% incubated with 10% inoculum and 200 mL /500 mL culture volume at 25℃ for 10 days.The symbiotic fungi from Crassostrea rivularis could be a new resource of anti-dementia substance.