为了实现木质纤维素浓醪酶解在低酶载量时的"三高"(高浓度、高转化率和高转化效率),通过利用常压甘油有机溶剂预处理甘蔗渣为底物,筛选合适的基质质量浓度(150 g/L)、纤维素酶添加量(6 FPU/g基质)和添加剂(吐温80,30 mg/g基质)。接着采用分批补料策略使基质质量浓度达到350 g/L,考察了不同加酶方式对分批补料浓醪酶解的影响。酶解72 h酶解液葡萄糖质量浓度达到132 g/L,葡萄糖转化率达到了理论值的60%。结果表明,常压甘油有机溶剂预处理基质具有较好的可酶解性,添加吐温80可以显著提高酶解效率。常压甘油有机溶剂预处理甘蔗渣的分批补料浓醪酶解推动了纤维素乙醇浓醪发酵工业化进程。

In order to reach the "THREE-HIGH"(High-titer,High-productivity and High-conversion) technical levels of enzymatic hydrolysisat low cellulase loading,the enzymatic hydrolysis at high solid concentration was conducted.The enzymatic hydrolysis conditions were optimized enzyme loading 6 FPU/g substrate,substrate concentration150 g/L) and the suitable additive(Tween 80 30 mg/g substrate.Further,a fed-batch strategy was adopted to make the final solid concentration up to 35%,followed by detecting a reasonable cellulase addition way.Finally,the final glucose yield achieved 132 g/L at 72 h from the fed-batch thick-mash enzymatic hydrolysis,with 60% of the cellulose conversion.Results indicate that the atmospheric alkali-catalyzed glycerol pretreated substrate very applicable and attractive for the thick-mash enzymatic hydrolysis at a low cellulase loading.The feedbatcha preferable way to realize "THREE-HIGH" technological level of thick-mash enzymatic hydrolysis of lignocellulosic biomass.