纳豆激酶(EC 3.4.21.62)是一种具有强大溶栓活性的丝氨酸蛋白酶。与临床一线溶栓药物(尿激酶和链激酶)相比,纳豆激酶具有安全性好、成本低、半衰期长和可口服等优点,作为溶栓药物或保健食品备受关注。纳豆激酶主要通过固态和液态发酵生产来制备,但现有生产能力偏低,远不能满足市场需求。文中从高产纳豆激酶的菌株选育、培养基优化、发酵条件优化和培养方式等4个方面对近年来相关研究进行了综述,以期为纳豆激酶的生产和应用提供支持。

Nattokinase(EC3.4.21.62) is a kind of serine proteinase that has potent thrombolytic activity.Compared to the first-line thrombolytic drugs(urokinase and streprokinase),nattokinase possesses several advantages such as safety,low cost,long half-life and easy oral administration.Therefore,nattokinase has been receiving increasing attention for its use as thrombolytic agent or health care product.The solid and liquid fermentation sare usually employed for the nattokinase production.However,the fermentation titer of nattokinase is still poor,and can meet the requirement of the market.In this review,the strain breeding,medium optimization,fermentation conditions optimization,and culture mode of nattokinase have been outlined and summarized,which would provide valuable reference for the industrial production and application of nattokinase.