利用响应面法优化扁桃果皮中熊果酸的最佳纯化工艺。为纯化扁桃果皮中的熊果酸,以吸附量与解析率为评价指标,从6种大孔树脂筛选出AB-8和HPD100两种最优树脂,按照一定质量比进行混合试验。在单因素试验基础上,根据Box-Benhnken实验原理设计响应面试验,得到优化纯化工艺为:上样液浓度0.31 mg/mL,上样液pH5.01,上样液流速1.0 mL/min;洗脱液浓度90%,洗脱液pH 5.06,洗脱液流速1.0 mL/min,在此条件下熊果酸的纯度由原来的6.35%提高到32%,提高了5.03倍。

Response surface methodology was used to optimize purification process of ursolic acid from Amygdalus communis L.peel.In order to purify ursolic acid in Amygdalus communis L.peel,adsorption mass and desorption rate were selected as the evaluation indexes,two resins of AB-8 and HPD100 were screened from six resins,and mixed with a certain mass ratio.On the basis of single factor experiments,response surface test was designed by BoxBenhnken experimental principle.The optimum purification process were as follows:sample concentration 0.31 mg/mL,sample pH 5.01,sample flow rate1.0 mL/min;eluent concentration 90%,eluent pH 5.06,eluent flow rate1.0 mL/min.Under the above conditions,ursolic acid purity increased 5.03 times which was from 6.35% to 32%.