为获得能利用葡萄糖/木糖混合糖液的高产油脂菌种,以野生酵母菌株Trichosporon dermatis ZZ-46为出发菌进行常压室温等离子体(atmospheric room temperature plasma,ARTP)-硫酸二乙酯(diethyl sulfate,DES)复合诱变,并分别以浅蓝菌素和氯化三苯基四氮唑(triphenyl tetrazolium chloride,TTC)分别为筛选因子结合尼罗红荧光染色进行高通量筛选,最终选育出1株高产菌株L7。该菌以混合糖为碳源进行摇瓶复筛后,生物量、油脂产量和油脂含量分别达到22.7 g/L、11.4 g/L和50.5%,较出发菌株分别提高12.6%、33.2%和7.8%;且该菌具有良好的遗传稳定性,其脂肪酸组成与出发菌相同,与植物油相似。结果表明,该菌具有较好利用混合糖液生产生物柴油的潜力。
To obtain a high-yield oil strain utilizing glucose and xylose, the wild yeast Trichosporon dermatis ZZ-46 was mutated by using atmospheric and room temperature plasma mutagenesis and diethyl sulfate treatment. A high-yield strain T. dermatis L7 was finally obtained after a high-throughput screening. With the mixed sugars used as a carbon source, the cell biomass, oil production, and oil content of this mutated strain reached 22.7 g/L, 11.4 g/L, and 50.5%, respectively, which were 12.6%, 33.2%, and 7.8% higher than that of parent strain, correspondingly. The mutant presented well genetic stability, and the fatty acid composition was the same as that of parent strain, which was extremely similar to vegetable oil. The mutant strain obtained in this study has potential in biodiesel production from lignocellulose hydrolysate predominately containing glucose and xylose.
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