以选育产S-腺苷蛋氨酸的高产酿酒酵母菌株为目标,利用常温常压等离子体和137Cs γ-射线对菌株进行诱变。通过5轮常温常压等离子体诱变和4轮γ-射线诱变,结合乙硫氨酸、制霉菌素抗性筛选,获得突变株AC-10,摇瓶发酵48 h,其S-腺苷蛋氨酸产量达到1.15 g/L,与出发菌株相比提高了130.0％。摇瓶发酵最适条件为30 ℃、初始pH 5.5。经5 L发酵罐分批补料发酵68 h,S-腺苷蛋氨酸产量达到5.62 g/L。
Atmospheric and room-temperature plasma (ARTP) mutagenesis and 137Cs γ-ray mutagenesis were used to mutate an S-adenosylmethionine (SAM)-producing Saccharomyces cerevisiae. After five runs of ARTP mutagenesis and four runs of γ-ray mutagenesis coupled with ethionine and nystatin-resistant screening, a high SAM producing mutant AC-10 was obtained. The SAM yield reached 1.15 g/L after 48 h flask fermentation, which was 130% higher than that of the original strain. The flask fermentation conditions were further optimized with the optimal temperature and initial pH of 30 ℃ and 5.5, respectively. The SAM titer by mutant AC-10 reached 5.62 g/L after 68 h in a 5 L fed-batch fermentation.
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