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二糖磷酸化酶及其在体外合成生物学中的应用

  • 李国玮 ,
  • 游淳
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  • 1(天津科技大学 生物工程学院,天津,300457);
    2(中国科学院天津工业生物技术研究所,天津,300308)
硕士研究生(游淳研究员为通讯作者,E-mail:you_c@tib.cas.cn)

收稿日期: 2020-06-02

  修回日期: 2020-06-16

  网络出版日期: 2020-12-11

基金资助

国家自然科学基金项目(21778073)

Disaccharide phosphorylase and its application in in vitro synthetic biology

  • LI Guowei ,
  • YOU Chun
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  • 1(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China);
    2(Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China)

Received date: 2020-06-02

  Revised date: 2020-06-16

  Online published: 2020-12-11

摘要

二糖磷酸化酶是以二糖为底物,催化磷酸向糖基转移,生成糖1-磷酸的一类酶。根据催化反应的立体化学过程可以分为保留型和翻转型,即供体底物二糖的异头构型可以在糖1-磷酸产物中保留或翻转。目前已发现的超过10种的二糖磷酸化酶均分布在糖苷水解酶和糖苷转移酶家族中。根据二糖磷酸化酶的功能设计体外生物合成体系,不仅可以用于制备高价值化学品,例如直链淀粉、肌醇、昆布二糖等,还可生产一些大宗产品,例如在氢能和酶燃料电池开发等方面已取得了进展。随着蛋白质工程的不断发展,对二糖磷酸化酶的研究越来越深入,相信未来二糖磷酸化酶将在体外生物合成平台扮演更重要的角色。

本文引用格式

李国玮 , 游淳 . 二糖磷酸化酶及其在体外合成生物学中的应用[J]. 食品与发酵工业, 2020 , 46(21) : 284 -291 . DOI: 10.13995/j.cnki.11-1802/ts.024623

Abstract

Disaccharide phosphorylases are a type of enzymes that utilize disaccharides as the substrate and catalyze the transfer of phosphate group to glycosyl to produce glucose 1-phosphates. According to the stereochemical course of the reaction catalyzed, disaccharide phosphorylases may be divided into retaining and inverting phosphorylases, according to the anomeric configuration of the donor disaccharides is retained or inverted in the resultant glucose 1-phosphate. At present, more than 10 disaccharide phosphorylases are discovered and distributed in the glycoside hydrolase and glycosyltransferase families. In vitro biosynthesis system designed based on the function of disaccharide phosphorylase can be used not only for the manufacturing of high-value chemicals, such as amylose, myo-inositol, and laminaribiose, but also for the production of bulk products, such as hydrogen energy and enzymatic fuel cells. With the development of protein engineering, disaccharide phosphorylase will play a significant role in in vitro biosynthesis.

参考文献

[1] CORI G,COLOWICK S,CORI C.The formation of glucose-1-phosphoric acid in extracts of mammalian tissues and of yeast[J].Journal of Biological Chemistry,1938,123(2):375-380.
[2] FISCHER E H.Cellular regulation by protein phosphorylation[J].Biochemical and Biophysical Research Communications,2013,430(2):865-867.
[3] COGAN E B,BIRRELL G B,GRIFFITH O H.A robotics-based automated assay for inorganic and organic phosphates[J].Analytical Biochemistry,1999,271(1):29-35.
[4] GAWRONSKI J,BENSON D.Microtiter assay for glutamine synthetase biosynthetic activity using inorganic phosphate detection[J].Analytical Biochemistry,2004,327(1):114-118.
[5] DE GROEVE M R M,TRAN G H,VAN HOOREBEKE A,et al.Development and application of a screening assay for glycoside phosphorylases[J].Analytical Biochemistry,2010,401(1):162-167.
[6] PUCHART V.Glycoside phosphorylases:Structure,catalytic properties and biotechnological potential[J].Biotechnology Advances,2015,33(2):261-276.
[7] KITAOKA M.Diversity of phosphorylases in glycoside hydrolase families[J].Applied Microbiology and Biotechnology,2015,99(20):8 377-8 390.
[8] O'NEILL E C,FIELD R A.Enzymatic synthesis using glycoside phosphorylases[J].Carbohydrate Research,2015,403:23-37.
[9] GIULIA P,SAKONWAN K,EESHAN K,et al.Glycan phosphorylases in multi-enzyme synthetic processes[J].Protein & Peptide Letters,2017,24(8):696-709.
[10] LAIRSON L,WITHERS S.Mechanistic analogies amongst carbohydrate modifying enzymes[J].Chemical Communications (Cambridge,England),2004,36(11):2 243-2 248.
[11] KAGAN B O,LATKER S N,Zfasman E M.Phosphorolysis of sucrose by the cultures of Leuconoctoc mesenteroides[J].Biokhimiya,1942,7:93-108.
[12] LULEY C,NIDETZKY B.Carbohydrate synthesis by disaccharide phosphorylases:Reactions,catalytic mechanisms and application in the glycosciences[J].Biotechnology Journal,2010,5(12):1 324-1 338.
[13] KITAMOTO Y,AKASHI H,TANAKA H,et al.α-Glucose-1-phosphate formation by a novel trehalose phosphorylase from Flammulina velutipes[J].Federation of European Microbiological Societies Microbiology Letters,1988,55(2):147-150.
[14] FITTING C,DOUDOROFF M.Phosphorolysis of maltose by enzyme preparation from Neisseria meningitidis[J].The Journal of Biological Chemistry,1952,199(1):153-163.
[15] EHRMANN M A,VOGEL R F.Maltose metabolism of Lactobacillus sanfranciscensis:cloning and heterologous expression of the key enzymes,maltose phosphorylase and phosphoglucomutase[J].Federation of European Microbiological Societies Microbiology Letters,1998,169(1):81-86.
[16] MARÉCHAL L R,BELOCOPITOW E.Metabolism of trehalose in euglena gracilis.I.Partial purification and some properties of trehalose phosphorylase[J].Journal of Biological Chemistry,1972,247(10):3 223-3 228.
[17] MARUTA K,MUKAI K,YAMASHITA H,et al.Gene encoding a trehalose phosphorylase from Thermoanaerobacter brockii ATCC 35047[J].Bioscience,Biotechnology,and Biochemistry,2002,66(9):1 976-1 980.
[18] CHAEN H,YAMAMOTO T,NISHIMOTO T,et al.Purification and characterization of a novel phosphorylase,kojibiose phosphorylase,from Thermoanaerobium brockii[J].Journal of Applied Glycoscience,1999,46(4):423-429.
[19] YAMAMOTO T,MARUTA K,MUKAI K,et al.Cloning and sequencing of kojibiose phosphorylase gene from Thermoanaerobacter brockii ATCC35047[J].Journal of Bioscience and Bioengineering,2004,98(2):99-106.
[20] NIHIRA T,NAKAI H,CHIKU K,et al.Discovery of nigerose phosphorylase from Clostridium phytofermentans[J].Applied Microbiology and Biotechnology,2012,93(4):1 513-1 522.
[21] NIHIRA T,MIYAJIMA F,CHIKU K,et al.One pot enzymatic production of nigerose from common sugar resources employing nigerose phosphorylase[J].Journal of Applied Glycoscience,2014,61(3):75-80.
[22] NIHIRA T,NAKAI H,KITAOKA M.3-O-α-d-Glucopyranosyl-l-rhamnose phosphorylase from Clostridium phytofermentans[J].Carbohydrate Research,2012,350:94-97.
[23] SIH C,McBee R H.A cellobiose phosphorylase in Clostridium thermocellum[J].Proceedings of the Montana Academy of Sciences,1955,15:21-22.
[24] REICHENBECHER M,LOTTSPEICH F,BRONNENMEIER K.Purification and properties of a cellobiose phosphorylase (CepA) and a cellodextrin phosphorylase (CepB) from the cellulolytic Thermophile clostridium stercorarium[J].European Journal of Biochemistry,1997,247(1):262-267.
[25] GOLDEMBERG S H,MARÉCHAL L R,De Souza B C.β-1,3-Oligoglucan:Orthophosphate glucosyltransferase from Euglena gracilis[J].Journal of Biological Chemistry,1966,241(1):45-50.
[26] KITAOKA M,MATSUOKA Y,MORI K,et al.Characterization of a bacterial laminaribiose phosphorylase[J].Bioscience,Biotechnology,and Biochemistry,2012,76(2):343-348.
[27] NIHIRA T,SAITO Y,KITAOKA M,et al.Characterization of a laminaribiose phosphorylase from Acholeplasma laidlawii PG-8A and production of 1,3-β-d-glucosyl disaccharides[J].Carbohydrate Research,2012,361:49-54.
[28] PARK J K,KEYHANI N O,Roseman S.Chitin catabolism in the marine bacterium Vibrio furnissii.identification,molecular cloning,and characterization of a N,N'-diacetylchitobiose phosphorylase[J].Journal of Biological Chemistry,2000,275(42):33 077-33 083.
[29] DERENSY-DRON D,KRZEWINSKI F,BRASSART C,et al.β-1,3-galactosyl-N-acetylhexosamine phosphorylase from Bifidobacterium bifidum DSM 20082:Characterization,partial purification and relation to mucin degradation[J].Biotechnology and Applied Biochemistry,1999,29(1):3-10.
[30] KITAOKA M,TIAN J,NISHIMOTO M.Novel putative galactose operon involving lacto-N-biose phosphorylase in Bifidobacterium longum[J].Applied and Environmental Microbiology,2005,71(6):3 158-3 162.
[31] NAKAJIMA M,NISHIMOTO M,KITAOKA M.Characterization of three beta-galactoside phosphorylases from Clostridium phytofermentans:Discovery of d-galactosyl-beta1->4-l-rhamnose phosphorylase[J].The Journal of Biological Chemistry,2009,284(29):19 220-19 227.
[32] NAKAJIMA M,NISHIMOTO M,KITAOKA M.Characterization of d-galactosyl-β-1→4-l-rhamnose phosphorylase from Opitutus terrae[J].Enzyme and Microbial Technology,2010,46(3):315-319.
[33] CHIKU K,NIHIRA T,SUZUKI E,et al.Discovery of two β-1,2-mannoside phosphorylases showing different chain-length specificities from Thermoanaerobacter sp.X-514[J].Public Library of Science One,2014,9(12):e11488.
[34] TSUDA T,NIHIRA T,CHIKU K,et al.Characterization and crystal structure determination of β-1,2-mannobiose phosphorylase from Listeria innocua[J].Federation of European Biochemical Societies Letters,2015,589(24):3 816-3 821.
[35] SENOURA T,ITO S,TAGUCHI H,et al.New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase[J].Biochemical and Biophysical Research Communications,2011,408(4):701-706.
[36] NIHIRA T,SUZUKI E,KITAOKA M,et al.Discovery of β-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase involved in the metabolism of N-glycans[J].The Journal of Biological Chemistry,2013,288(38):27 366-27 374.
[37] MURAO S,NAGANO H,OGURA S,et al.Enzymatic synthesis of trehalose from maltose[J].Agricultural and Biological Chemistry,1985,49(7):2 113-2 118.
[38] AERTS D,VERHAEGHE T,ROMAN B,et al.Transglucosylation potential of six sucrose phosphorylases toward different classes of acceptors[J].Carbohydrate Research,2011,346(13):1 860-1 867.
[39] MORIMOTO K,YOSHIHARA A,FURUMOTO T,et al.Production and application of a rare disaccharide using sucrose phosphorylase from Leuconostoc mesenteroides[J].Journal of Bioscience and Bioengineering,2014,119(6):652-656.
[40] ICHIKAWA M,SCHNAAR R L,ICHIKAWA Y.Application of sucrose phosphorylase reaction in one-pot enzymatic galactosylation:Scavenger of phosphate and generation of glucose 1-phosphate in situ[J].Tetrahedron Letters,1995,36(48):8 731-8 732.
[41] WILDBERGER P,PFEIFFER M,BRECKER L,et al.Diastereoselective synthesis of glycosyl phosphates by using a phosphorylase-phosphatase combination catalyst[J].Angewandte Chemie International Edition in English,2015,54(52):15 867-15 871.
[42] You C,Chen H,Myung S,et al.Enzymatic transformation of nonfood biomass to starch[J].Proceedings of the National Academy of Sciences of the United States of America,2013,110(18):7 182-7 187.
[43] MENG D,WEI X,ZHANG Y-H P J,et al.Stoichiometric conversion of cellulosic biomass by in vitro synthetic enzymatic biosystems for biomanufacturing[J].American Chemical Society Catalysis,2018,8(10):9 550-9 559.
[44] ZHONG C,YOU C,WEI P,et al.Thermal cycling cascade biocatalysis of myo-inositol synthesis from sucrose[J].American Chemical Society Catalysis,2017,7(9):5 992-5 999.
[45] SUN S,WEI X,YOU C.The construction of an in vitro synthetic enzymatic biosystem that facilitates laminaribiose biosynthesis from maltodextrin and glucose[J].Biotechnology Journal,2019,14(4):1 800 493.
[46] YE X,WANG Y,HOPKINS R C,et al.Spontaneous high-yield production of hydrogen from cellulosic materials and water catalyzed by enzyme cocktails[J].Chem Sus Chem,2009,2(2):149-152.
[47] MYUNG S,ROLLIN J,YOU C,et al.In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose[J].Metabolic Engineering,2014,24:70-77.
[48] 庞海丽. 直接醇类燃料电池和酶生物燃料电池电极研究[D].长沙:湖南大学,2011.
[49] ZHU Z,MA C,ZHANG Y H P.Co-utilization of mixed sugars in an enzymatic fuel cell based on an in vitro enzymatic pathway[J].Electrochimica Acta,2017,263:184-191.
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