纳米硒具有毒性低而生物活性强的特征,在医药、保健品领域具有巨大的市场竞争力,生物制备纳米硒在国内研究较少。从湖北省恩施州富硒矿区土壤中成功分离了1株能将亚硒酸钠还原为红色产物的菌株,对该菌株进行了亚硒酸钠耐受性、形态及分类上的分析;对红色发酵产物进行了分离纯化及电镜、X射线光电子能谱法 (X-ray photoelectron spectroscopy,XPS)分析。电镜结果显示,该菌呈杆状,16S rRNA测序及MEGA 6.0建树分析表明该菌属于假单胞菌(Pseudomonas),对亚硒酸钠的耐受性为10~20 mg/mL;红色物质经蔗糖密度梯度离心纯化后鉴定为纳米硒,粒径在100~200 nm。该假单胞菌可作为生产红色纳米硒的菌株,可为硒的生物转化提供参考。
Nano-selenium has low toxicity and strong biological activity,therefore,it has great attraction and competitiveness in the market of medicine and health care products.A strain able to reduce selenite to red product was successfully isolated from the soil of selenium-rich mining area in Enshi Tujia and Miao autonomous prefecture,Hubei province.The selenite tolerance,morphology and taxonomy of the strain were analyzed,and the red fermentation product was purified and analyzed by electron microscopy and X-ray photoelectron spectroscopy (XPS).The results of electron microscopy showed that the strain was rod-shaped.The phylogenetic tree analysis showed that the strain was attributed to Pseudomonasand the tolerance of selenite ranged from 10 to 20 mg/mL.The red substance was identified as nano-selenium,the size ranged from 100 to 200 nm.The Pseudomonassp.has the potential for the production of red nano-selenium.
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