为解析桃果采后内生细菌群落结构组成及多样性,挖掘其中潜在的益生微生物资源。该文利用高通量测序技术对新疆不同品种的桃果内生细菌进行扩增与分析。结果表明,3类桃果内生细菌共包括106个操作分类单元(operational taxonomic units,OTU),涉及9个门,59个属,包含73个相同OTUs。进一步分析发现,水蜜桃中主要菌群为泛菌属和芽孢杆菌属,其中泛菌属菌群数占到总数的1/3以上;蟠桃中主要菌群为假单胞菌属和未分类菌属,约占总体的19.83%、16.57%。油桃中主要菌群为芽孢杆菌属和未分类属,约占总体的15.63%、15.15%;不动杆菌属(Acinetobacter)、拟杆菌属(Bacteroides)为油桃、水蜜桃和蟠桃的共同优势菌群,分别约占总体的7.41%、10.58%、14.49%和8.65%、4.30%、8.91%。同时,研究发现双歧杆菌属(Bifidobacterium)、乳酸杆菌属(Lactobacillus)、阿卡曼氏属(Akkermansia)等与人体健康相关的菌群大量存在于桃果中。此外,桃果中存在大量未知分类菌群。该文为进一步分析桃果内生细菌群落与人体健康潜在的相互关系,深度挖掘与利用内生细菌资源研究提供了研究基础。
In order to analyze the composition and diversity of endophytic bacteria communities and explore the potential probiotics resources in peaches,high-throughput sequencing was performed to amplify and analyze the endophytic bacteria communities from different Xinjiang peaches.The results showed that 106 operational taxonomic units (OTUs) including 59 genera in 9 phyla were obtained,in which 73 OTUs co-existed in these three kinds of peaches.Pantoea and Bacillus were the major genera in honey peach,of which Pantoea accounted for more than 1/3 of the total.The main genera in peento were Pseudomonas and unclassified bacteria,which accounted for 19.83% and 16.57%,respectively.While Bacillus and unclassified bacteria were found densely in the nectarine accounting for 15.63% and 15.15%,respectively.Additionally,Acinetobacter and Bacteroides were the dominant bacteria in these three kinds of peaches,accounting for 7.41%,10.58%,14.49% and 8.65%,4.30%,8.91% of the total respectively.Furthermore,many endophytic bacteria in Bifidobacterium,Lactobacillus and Akkermansia related to human health were observed,and many unclassified endophytic bacteria were detected in peaches.It provides a scientific basis to analysis the potential relationship between endophytic bacteria and human health,and to utilize the resources of endophytic bacteria in peaches.
[1] 邢震.保鲜剂处理结合冰温对大久保桃采后品质劣变的影响研究[D].石河子:石河子大学,2010.
[2] 陈健.五果之首属鲜桃[J].健康指南:医疗保健服务,2016(9):38-39.
[3] WANG Y,LIN D,WANG X,et al.The impact of a novel peach gum-derived polysaccharide on postprandial blood glucose control in streptozotocin-induced diabetic mice[J].International Journal of Biological Macromolecules,2017,98:379-386.
[4] 卢娟芳,刘盛雨,芦旺,等.不同类型桃果肉酚类物质及抗氧化活性分析[J].中国农业科学,2017,50(16):3 205-3 214.
[5] SATTAR S,IMRAN M,MUSHTAQ Z,et al.Functional quality of optimized peach-based beverage developed by application of ultrasonic processing[J].Food Science &Nutrition,2019,7(11):3 692-3 699.
[6] 克里木·伊明,韩立群,玛尔哈巴·吾斯曼,等.新疆桃果实性状调查与评价研究[J].新疆农业科学,2017,54(6):1 041-1 046.
[7] 邰晓亮.不同贮藏条件对蟠桃采后生理及贮藏效果影响的研究[D].石河子:石河子大学,2010.
[8] WANG Q,WEI Y,CHEN X,et al.Postharvest strategy combining maturity and storage temperature for 1-MCP-treated peach fruit[J].Journal of Food Processing and Preservation,2020,44(4):e14 388.
[9] ZHOU D,WEI Y,PENG J,et al.Carvacrol and eugenol inhibit postharvest soft rot disease by enhancing defense response in peaches during storage[J].Journal of Food Processing and Preservation,2019,43(9):e14 086.
[10] 黄敬瑜,张楚军,姚瑜龙,等.植物内生菌生物抗菌活性物质研究进展[J].生物工程学报,2017,33(2):178-186.
[11] 程亮.青藏高原矮火线草内生细菌多样性及生物功能分析[J].南方农业学报,2019,50(10):2 222-2 233.
[12] 张猛,王琼,冯发运,等.植物内生特基拉芽胞杆菌的分离、鉴定及防治西瓜枯萎病效果[J].中国生物防治学报,2017,33(3):371-377.
[13] 王玉霞,李唯,王旺田,等.植物果实特异性启动子E8基因的克隆[J].华北农学报,2008,23(3):16-19.
[14] 木尼热木·阿力木江,楚敏,唐琦勇,等.辐射污染区盐爪爪根际可培养细菌群落组成及功能特性[J].微生物学通报,2018,45(7):1 416-1 425.
[15] 申艳红,姜涛,刘璐琪,等.基于高通量测序发掘番木瓜果实成熟相关miRNA[J].果树学报,2019,36(1):1 473-1 482.
[16] 王梦颖.香蕉内生菌多样性分析及广谱拮抗菌筛选[D].海口:海南大学,2014.
[17] 蔡学清,陈炜,林娜,等.内生细菌在荔枝体内的定殖及其防病保鲜功能[J].应用生态学报,2011,22(8):2 140-2 146.
[18] 蔡学清,林娜,陈炜,等.内生细菌对荔枝果实营养成分的影响[J].福建农林大学学报(自然科学版),2010,39(3):236-240.
[19] 许日升,张伟,苏春沦,等.花生内生菌及其应用研究进展[EB/OL].北京:中国科技论文在线.[2015-02-09].http://www.paper.edu.cn/releasepaper/content/4631609.
[20] 周克茹.n-3多不饱和脂肪酸对肠道菌作用线虫模型的建立与应用[D].无锡:江南大学,2017.
[21] KRAUTKRAMER K,KREZNAR J,ROMANO K A,et al.Diet-microbiota interactions mediate global epigenetic programming in multiple host tissues[J].Molecular Cell,2016,64(5):982-992.
[22] HOODA S,BOLER B M V,KERR K R,et al.The gut microbiome of kittens is affected by dietary protein:Carbohydrate ratio and associated with blood metabolite and hormone concentrations[J].British Journal of Nutrition,2013,109(9):1 637-1 646.
[23] 郭虹雯,许翔雨,陈莹婕,等.绿茶茶汤对肥胖相关肠道菌群的影响[J].茶叶科学,2016,36(4):354-362.
[24] 黄菊青,林斌,徐庆贤,等.绞股蓝茶饮对高脂血症大鼠脂质代谢和肠道菌群的影响[J].中国食品学报,2018,18(6):27-32.
[25] MARCO T,ROBERTA D G I,LAURA S,et al.Effect of Lactobacillus rhamnosus HN001 and Bifidobacterium longum BB536 on the healthy gut microbiota composition at phyla and species level:A preliminary study[J].World Journal of Gastroenterology,2017,23(15):2 696-2 704.
[26] DAO M C,EVERARD A,ARON-WISNEWSKY J,et al.Akkermansia muciniphila and improved metabolic health during a dietary intervention in obesity:Relationship with gut microbiome richness and ecology[J].Gut Microbiota,2016,65(3):426-436.
[27] 秦红,蔡传江,赵燕,等.酿酒酵母和芽孢杆菌对育肥猪养分表观消化率、肠道形态结构和肠道免疫的影响[J].动物营养学报,2017,29(12):4 459-4 468.
[28] 江向洋.肠道菌群与NAFLD发生发展的关联代谢机制研究[D].杭州:浙江大学,2017.
