苦瓜青钱柳复合茶(后文简称复合茶)不仅可以抑制活性氧和老年斑的积累,改善神经保护作用,还可以减轻脂肪的积累。进一步利用秀丽隐杆线虫(Caenorhabditis elegans)模型研究其抗氧化应激活性,为进一步推广和应用提供了理论依据。首先,复合茶可以为暴露于H2O2和百草枯引起的氧化应激的线虫提供很好的保护作用,尤其对氧化应激敏感的突变体TK22。进一步探索其抗氧化特性,发现线虫的抗氧化防御体系显著被复合茶激活。此外,复合茶处理显著提升了线虫抗氧化酶基因(sod-3、sod-5、ctl-1和ctl-2)和抗逆基因(hsp-16.1和hsp-16.2)的表达。最后,复合茶不会损害线虫的繁殖和发育,甚至缓解了移动力的衰退。该研究为复合茶作为膳食佐剂在氧化应激管理中提供了理论依据,并通过寻求新的创新应用为青钱柳及苦瓜增加了价值。
A compoundMomordica charantia andCyclocarya paliurus tea (compound tea) was found to not only inhibit the accumulation of reactive oxygen species and age pigments and improve neuroprotection,but also alleviate fat accumulation in previous study.The model of Caenorhabditis elegans was used to further study its antioxidative stress activity,which provided a theoretical basis for further promotion and application.First,it provided the protection toC.elegans exposed to H2O2-and paraquat-induced oxidative stress,especially in the mutant TK22 sensitive to oxidative stress.Further exploration of its antioxidant properties revealed that the antioxidant defense system ofC.elegans was significantly activated by compound tea.In addition,the expression of antioxidant enzyme genes (sod-3,sod-5,ctl-1,andctl-2) and stress resistance genes (hsp-16.1 andhsp-16.2) were also significantly increased.Finally,it was found that y did not damage the reproduction and development ofC.elegans,and even alleviated the decline of mobility.Overall,these results provided promising data for compound tea as a dietary adjuvant in oxidative stress management,and added value toMomordica charantia andCyclocarya paliurus by seeking new and innovative applications.
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