食品与发酵工业 >

2020 , Vol. 46 >Issue 23: 184 - 192

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024733

生产与科研应用

苦竹笋总黄酮大孔树脂纯化工艺及其体外抗炎活性研究

  • 晏俊玲 ,
  • 樊扬 ,
  • 秦川 ,
  • 欧雪 ,
  • 陈姝娟 ,
  • 敖晓琳
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  • (四川农业大学 食品学院,四川 雅安,625014)
硕士研究生(敖晓琳副教授为通讯作者,E-mail:huavslin@163.com)

收稿日期: 2020-06-12

  修回日期: 2020-07-05

  网络出版日期: 2020-12-30

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Purification of total flavonoids from bamboo shoots of Pleioblastus amarus with macroporous resin and its anti-inflammation activity

  • YAN Junling ,
  • FAN Yang ,
  • QIN Chuan ,
  • OU Xue ,
  • CHEN Shujuan ,
  • AO Xiaolin
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  • (College of Food Science,Sichuan Agricultural University,Ya'an 625014,China)

Received date: 2020-06-12

  Revised date: 2020-07-05

  Online published: 2020-12-30

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摘要

采用大孔吸附树脂对苦竹笋总黄酮进行纯化,探索其最佳工艺条件,并对其富集产物进行体外抗炎活性测定。首先对树脂的筛选、吸附动力学、吸附等温线和热力学进行了研究,再进行动态吸附和解吸试验,筛选得出AB-8是最适宜纯化苦竹笋总黄酮的树脂。准二级动力学模型和Langmuir等温线模型对吸附数据进行了最佳描述,吸附过程自发、放热。确定纯化最优工艺为:上样量4.2 BV(105 mL)、上样液质量浓度 1.55 mg/mL、上样流速1 BV/h、上样液pH值为7、洗脱剂为50%乙醇溶液(体积分数)、洗脱剂用量8 BV(200 mL)、洗脱流速4 BV/h,在此工艺条件下,总黄酮回收率可达到75.03%,总黄酮含量由原来的2.46%提高至15.72%。且纯化后的苦竹笋总黄酮表现出更好的NO抑制作用,纯化样品总黄酮浓度达到1.04 mg/mL抑制率可达到97.80%(NO浓度为5.60 μmol/L),而同一总黄酮浓度下的粗提样品中NO浓度为13.81 μmol/L,抑制率仅为73.22%。综上,该实验建立的苦竹笋总黄酮的纯化方法有效可行。

关键词： 苦竹笋; 总黄酮; 大孔吸附树脂; 吸附动力学; 吸附热力学; 抗炎活性

本文引用格式

晏俊玲 , 樊扬 , 秦川 , 欧雪 , 陈姝娟 , 敖晓琳 . 苦竹笋总黄酮大孔树脂纯化工艺及其体外抗炎活性研究[J]. 食品与发酵工业, 2020 , 46(23) : 184 -192 . DOI: 10.13995/j.cnki.11-1802/ts.024733

Abstract

The aim of this work is to develop an efficient and economical method for the enrichment of total flavonoids from the shoots ofP.amarus,and to determine its anti-inflammatory activity in vitro.Before the dynamic adsorption and desorption tests,the resin screening,adsorption kinetics,adsorption isotherm and thermodynamics were studied successively.The screening showed that AB-8 resin is the most suitable resin for purifying the total flavonoids of the shoots ofP.amarus.The static experimental data correlated well with the pseudo-second-order kinetics model and Langmuir isotherm model.The adsorption process is spontaneous and exothermic.The optimum enrichment conditions were as follows:for adsorption the total flavonoids concentration,flow rate and volume of sample were 1.55 mg/mL,1 BV/h and 4.2 BV.Respectively,and for desorption the flavonoids-loaded AB-8 resin column was desorbed by 8 BV of 50% ethanol at a rate of 4 BV/h,the recovery yield of total flavonoids was 75.03%,total flavonoids content increased from 2.46% to 15.72%.The total flavonoids of the purified could better inhibit the production of NO.When the total flavonoids concentration reached 1.04 mg/mL,the inhibition rate reached 97.80%(the concentration of NO was 5.60 mol/L),while the concentration of NO in the crude extracts was 13.81 mol/L,inhibition rate was only 73.22%.In summary,the purification method of total flavonoids of the shoots ofP.amarus established in this experiment is effective and feasible.

Key words： Pleioblastus amarus; total flavonoids; macroporous adsorption resin; adsorption kinetics; adsorption thermodynamics; anti-inflammatory activity

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