食品与发酵工业 >

2021 , Vol. 47 >Issue 1: 21 - 26

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025545

研究报告

转化虎杖苷虎杖内生真菌的分离及鉴定

  • 于洁 ,
  • 徐勤茜 ,
  • 李子院 ,
  • 刘红艳 ,
  • 郝再彬 ,
  • 李海云
展开
  • (桂林理工大学 化学与生物工程学院,广西高校食品安全与检测重点实验室,广西 桂林,541004)
硕士研究生(李海云教授为通讯作者,E-mail:xglihaiyun@126.com)

收稿日期: 2020-09-01

  修回日期: 2020-09-13

  网络出版日期: 2021-02-03

基金资助

国家自然科学基金项目(31460409);广西自然科学基金项目(2016GXNSFAA380014);广西科技基地和人才专项项目(桂科AD18050004);广西壮族自治区特聘专家项目(农产品精深加工关键技术与质量安全,厅发[2018]39)

收起

Isolation and identification of endophytic fungi from Polygonum cuspidatum for polydatin transformation

  • YU Jie ,
  • XU Qinqian ,
  • LI Ziyuan ,
  • LIU Hongyan ,
  • HAO Zaibin ,
  • LI Haiyun
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  • (College of Chemistry & Bioengineering, Guangxi Colleges and Universities, Key Laboratory of Food Safety and Detection, Guilin University of Technology, Guilin 541004, China)

Received date: 2020-09-01

  Revised date: 2020-09-13

  Online published: 2021-02-03

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摘要

为寻找可高效转化虎杖苷生成白藜芦醇的微生物新菌株,采用以虎杖苷为唯一碳源的选择性培养平板,从中药虎杖中分离筛选出4株生长良好的内生真菌,分别编号为HZ001、HZ002、HZ003和HZ004;考察了4菌株细胞和发酵液转化虎杖苷的性能,并对菌株进行了形态学和分子生物学鉴定。菌体细胞生物转化实验结果表明,HZ001和HZ004可有效转化虎杖苷生成白藜芦醇,摩尔转化产率均可达100%,而HZ002和HZ003菌体细胞转化体系中未检测到白藜芦醇;发酵液生物转化实验结果表明,HZ001、HZ003、HZ004发酵液转化虎杖苷生成白藜芦醇的摩尔转化产率分别为55%、40%和38%。结果表明,HZ001和HZ004转化虎杖苷生成白藜芦醇的性能最强,其主要靠胞内酶发挥作用。菌株鉴定结果表明,HZ001和HZ002分别归属为Aspergillus aculeatus和Penicillium georgiense,HZ003和HZ004则均归属为Aspergillus flavus。

关键词： 虎杖; 内生真菌; 虎杖苷; 白藜芦醇; 生物转化

本文引用格式

于洁 , 徐勤茜 , 李子院 , 刘红艳 , 郝再彬 , 李海云 . 转化虎杖苷虎杖内生真菌的分离及鉴定[J]. 食品与发酵工业, 2021 , 47(1) : 21 -26 . DOI: 10.13995/j.cnki.11-1802/ts.025545

Abstract

In order to obtain new microbial strains that can efficiently transform polydatin into resveratrol, four endophytic fungi, numbered as HZ001, HZ002, HZ003 and HZ004 were isolated from Polygonum cuspidatum by selective media using polydatin as the sole carbon source. The activity of four strains of cells and fermentation broth to transform polydatin were identified, and the morphological and molecular biological identification of the strains were carried out. The results of cell transformation assays showed that, polydatin could be transformed into resveratrol by cells of HZ001 and HZ004 with the molar conversion rates of 100%, while no resveratrol was detected in HZ002 and HZ003 cell transformation systems. The results of fermentation broth biotransformation assays showed that polydatin could be transformed into resveratrol by fermentation broths of HZ001, HZ003 and HZ004 with the conversion rates of 55%, 40% and 38%, respectively. The results indicated that HZ001 and HZ004 had the strongest activities to convert polydatin into resveratrol, which mainly depended on the intracellular enzymes. The results of morphological and molecular biological identification indicated that HZ001 and HZ002 were attributed to Aspergillus aculeatus and Penicillium georgiense respectively, both HZ003 and HZ004 were attributed to Aspergillus flavus.

Key words： Polygonum cuspidatum; endophytic fungi; polydatin; resveratrol; biotransformation

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