为探明不同品种月季花瓣营养成分间的差异和相关性,该研究对河南南阳地区10个不同栽培品种月季花瓣中的基本营养成分进行了测定,并结合化学计量学对各营养成分进行了分析评价。结果表明,所测品种中蛋白质和脂肪的平均含量分别为8.93%、1.28%(质量分数);矿物元素K、Ca、Mg、Na、Fe、Mn、Cu、Zn、Se等的平均含量分别为14 459.41、2 171.43、1 692.21、53.04、93.31、30.98、3.70、20.89、0.87 μg/g,且品种间差异达显著水平;主成分分析得出4因子模型,提取前4个主成分其累计贡献率达到 83.24%,主成分分析和聚类分析均将10个品种的月季花划分为五类,表明这2种分析法可以很好地区分不同月季花品种。该研究为月季花种质的筛选、品质评价和月季花资源的综合利用和产品开发提供了一定的科学参考。
To investigate the correlation between the nutrient contents of different species of Chinese rose petals,Kjeldahl nitrogen determination,inductively coupled plasma atomic emission spectroscopy (ICP-AES),and acid hydrolysis were employed to measure the principal nutrient contents in ten species of Chinese rose petals from Nanyang in Henan.Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were then utilized to evaluate each nutrient.Using these methods,the average protein and fat levels across ten different species were 8.93% and 1.28%,respectively.The average contents of mineral elements K,Ca,Mg,Na,Fe,Mn,Cu,Zn,Se were 14 459.41,2 171.43,1 692.21,53.04,93.31,30.98,3.70,20.89,0.87 μg/g.However,there were statistically significant differences in the concentration of protein,fat,macroelements,and microelements across species.Moreover,the coefficient of variances of difference species were significantly different.PCA of each nutrient content resulted in a four-factor model,and the cumulative contribution rate of the four principal components was 83.24%.The ten species were categorized into 5 groups by PCA,which was consistent with the result obtained using HCA and indicating that PCA and HCA were suitable methods for classification of different Chinese rose species.The study thus provides scientific references and valuable insights for germ plasm screening,quality evaluation,integrated utilization,and product development of Chinese roses.
[1] 中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学技术出版社,1985,37:361.
Editorial Committee of Chinese Flora of Chinese Academy of Sciences.Flora Reipublicae Popularis Sinicae[M].Beijing:Science and Technology Press,1985,37:361.
[2] 国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2020.
National Pharmacopoeia Committee.Pharmacopoeia of the People′s Republic of China[M].Beijing:China Medical Science and Technology Press,2020.
[3] 钱慧琴,秦晶晶,赵媛,等.月季不同部位总黄酮含量分析及其抗氧化活性研究[J].食品研究与开发,2018,39(20):19-22.
QIAN H Q,QIN J J,ZHAO Y,et al.Antioxidant activities and contents of total flavonoids from different parts of Rosa chinensis Jacq[J].Food Research And Development,2018,39(20):19-22.
[4] ZHOU W,ZHANG Y,XU H,et al.Determination of elemental composition of volatile organic compounds from Chinese rose oil by spectral accuracy and mass accuracy[J].Rapid Communications in Mass Spectrometry,2011,25(20):3 097-3 102.
[5] SALMINEN A,LEHTONEN M,SUURONEN T,et al.Terpenoids:natural inhibitors of NF-kappa B signaling with anti-inflammatory and anticancer potential[J].Cellular and Molecular Life Sciences,2008,65(19):2 979-2 999.
[6] ASRES K,SEYOUM A,VEERESHAM C,et a1.Naturally derived anti-HIV agents[J].Phytotherapy Research,2005,19(7):557-581.
[7] CAI Y Z,XING J,SUN M,et al.Phenolic antioxidants (hydrolyzable tannins,flavonols,and anthocyanins) identified by LC-ESI-MS and MALDI-QIT-TOF MS from Rosa chinensis flowers[J].Journal of Agricultural and Food Chemistry,2005,53(26):9 940-9 948.
[8] 于佳.月季花抗衰老化妆品的开发研究[D].天津:天津大学,2015.
YU J.Development and study of Rosa chinensis extract in anti-aging cosmetics[D].Tianjin:Tianjin University,2015.
[9] 蔡元元.月季花正丁醇部位的化学成分及抗肿瘤活性的研究[D].郑州:郑州大学,2014.
CAI Y Y.Study oil chemical constituents and anti-tumor activity of the n-BuOH fraction from Flos Rosa chinensis[D].Zhengzhou:Zhengzhou University,2014.
[10] 刘英发,王宪明,王敏伟.月季花水提物对外源性一氧化氮损伤的胰岛细胞的保护作用[J].沈阳药科大学学报,2006,23(2):109-112.
LIU Y F,WANG X M,WANG M W.Protective effect of water extracts of Flos Rosae Chinensis on B-cell injury induced by exogenous nitric oxide[J].Journal of Shenyang Pharmaceutical University,2006,23(2):109-112.
[11] 李艳,宋亚刚,苗明三,等.月季花总黄酮对局灶性脑缺血再灌注模型大鼠的影响[J].中国实验方剂学杂志,2019,25(12):64-70.
LI Y,SONG Y G,MIAO M S,et al.Effect of Rosae Chinensis Flos total flavones on focal cerebral ischemia-reperfusion in rats[J].Chinese Journal of Experimental Traditional Medical Formulae,2019,25(12):64-70.
[12] 王芳,杨永莉.可食用花卉-月季营养成分分析[J].山西农业大学学报(自然科学版),2006,(2):183-185.
WANG F,YANG Y L.Nutrient analysis of edible Rosa Chinensis[J].Journal of Shanxi Agricultural University (Natural Science Edition),2006,(2):183-185.
[13] 茹宗玲,彭聪虎.月季花黄色素的稳定性研究[J].食品研究与开发,2009,30(8):189-192.
RU Z L,PENG C H.Study on the stability of yellow pigment in Rosa Chinensis[J].Food Research and Development,2009,30(8):189-192.
[14] 张敏娜,王光辉,丁瑞芳,等.月季花挥发油的提取及其β-环糊精包合物的初步研究[J].上海医药,2019,40(1):62-65.
ZHANG M N,WANG G H,DING R F,et al.Extraction of volatile oil from Rosa Chinensis Jacq and primary study on the inclusion compound of β-cyclodextrin[J].Shanghai Medical Journal,2019,40(1):62-65.
[15] 宋华静,吴荣书.食用花卉的保健价值及发展前景[J].中国食品工业,2009(3):60-61.
SONG H J,WU R S.The health value and development prospect of edible flowers[J].China Food Industry,2009(3):60-61.
[16] 邵小斌,赵统利,朱朋波,等.主要切花月季品种评价与筛选[J].浙江农业科学,2019,60(11):2 063-2 065.
SHAO X B,ZHAO T L,ZHU P B,et al.Evaluation and selection of main cut rose varieties[J].Journal of Zhejiang Agricultural Science,2019,60(11):2 063-2 065.
[17] 于海超,刘刚,马殿旭,等.不同月季花品种的FTIR研究[J].激光与光电子学进展,2015,52(7):284-288.
YU H C,LIU G,MA D X,et al.Study of different Chinese rose varieties by FTIR[J].Laser &Optoelectronics Progress,2015,52(7):284-288.
[18] 中华人民共和国.GB 5009.5—2016 食品安全国家标准 食品中蛋白质的测定[S].北京:中国标准出版社,2017.
The People′s Republic of China.GB 5009.5—2016 National Food Safety Standard Determination of protein in food[S].Beijing:China Standards Press,2017.
[19] 中华人民共和国.GB 5009.6—2016 食品安全国家标准 食品中脂肪的测定[S].北京:中国标准出版社,2017.
The People′s Republic of China.GB 5009.5—2016 National Food Safety Standard Determination of fat in food[S].Beijing:China Standards Press,2017.
[20] 张孟琴,孙亚真,范一霖.牡丹花粉营养成分评价及相关性分析[J].食品研究与开发,2018,39(15):154-156;160.
ZHANG M Q,SUN Y Z,FAN Y L.Evaluation and correlation analysis on nutrition contents of pollen from six different species Paeonia suffruticosa[J].Food Research and Development,2018,39(15):154-156;160.
[21] 于秀林,任雪松.多元统计分析[M].北京:中国统计出版社,1995.
YU X L,REN X S.Multivariate Statistical Analysis[M].Beijing:China Statistics Press,1995.
[22] 张龙进,李桂双,白成科,等.山茱萸种质资源数量性状评价及相关性分析[J].植物遗传资源学报,2012,13(4):655-659.
ZHANG L J,LI G S,BAI C K,et al.Evaluation and correlation analysis on quantitative traits of Cornus officinalis germplasm resources[J].Journal of Plant Genetic Resources,2012,13(4):655-659.